Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Effects of high-energy-pulse-electron beam radiation on biomacromolecules | |
| Zhu, H; Xu, JZ; Li, SQ; Sun, XY; Yao, SD（姚思德）; Wang, SL; Wang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China | |
| 2008 | |
| Source Publication | SCIENCE IN CHINA SERIES B-CHEMISTRY
 |
| ISSN | 1006-9291 |
| Volume | 51Issue:1Pages:86 |
| Abstract | To study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by Co-60 gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future. |
| Subject Area | Computer Science ; Engineering ; Robotics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000251972200013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7709 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Wang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, H,Xu, JZ,Li, SQ,et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(1):86. |
| APA | Zhu, H.,Xu, JZ.,Li, SQ.,Sun, XY.,Yao, SD.,...&Wang, SL .(2008).Effects of high-energy-pulse-electron beam radiation on biomacromolecules.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(1),86. |
| MLA | Zhu, H,et al."Effects of high-energy-pulse-electron beam radiation on biomacromolecules".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.1(2008):86. |
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