CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Effects of high-energy-pulse-electron beam radiation on biomacromolecules
Zhu, H; Xu, JZ; Li, SQ; Sun, XY; Yao, SD(姚思德); Wang, SL; Wang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
2008
Source PublicationSCIENCE IN CHINA SERIES B-CHEMISTRY
ISSN1006-9291
Volume51Issue:1Pages:86
AbstractTo study the molecular mechanism of high mutation frequency induced by high-energy-pulse-electron (HEPE) beam radiation, the effects of HEPE radiation on yeast cells, plasma membrane, plasmid DNA, and protein activity were investigated by means of cell counting, gel electrophoresis, AO/EB double fluorescent staining, etc. The results showed that the viability of yeast cells declined statistically with increase of absorbed doses. The half lethal dose (LD50) was 134 Gy. HEPE beam radiation had little influence on the function of plasma membrane and protein, while it could induce much DNA damage of single strand breaks (SSB) and double strand breaks (DSB) that were required for gene mutation. The G-value for DSB formation of HEPE beam radiation in aqueous solution was 5.7 times higher than that caused by Co-60 gamma rays. HEPE can be a new effective method for induced mutation breeding and deserves further research in the future.
Subject AreaComputer Science ; Engineering ; Robotics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000251972200013
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7709
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorWang, SL (reprint author), Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
Recommended Citation
GB/T 7714
Zhu, H,Xu, JZ,Li, SQ,et al. Effects of high-energy-pulse-electron beam radiation on biomacromolecules[J]. SCIENCE IN CHINA SERIES B-CHEMISTRY,2008,51(1):86.
APA Zhu, H.,Xu, JZ.,Li, SQ.,Sun, XY.,Yao, SD.,...&Wang, SL .(2008).Effects of high-energy-pulse-electron beam radiation on biomacromolecules.SCIENCE IN CHINA SERIES B-CHEMISTRY,51(1),86.
MLA Zhu, H,et al."Effects of high-energy-pulse-electron beam radiation on biomacromolecules".SCIENCE IN CHINA SERIES B-CHEMISTRY 51.1(2008):86.
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