CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin
Wang, J; Liang, ZQ; Wang, LH; Fan, CH; Li, GX; Li, GX (reprint author), Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
2007
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume125Issue:1Pages:17
AbstractA simple and novel strategy to obtain the direct electrochemistry of hemoglobin (Hb) was reported by enhancing its structural rigidity, which was demonstrated by the great promotion of the electron-transfer reactivity of the protein and its catalytic activity toward hydrogen peroxide (H2O2). Direct fast electron transfer between this structural rigidity enhanced Hb (SRE Hb) and a pyrolytic graphite (PG) electrode was achieved, and a pair of well-defined, quasi-reversible redox peaks were obtained. The cathodic and anodic peaks were located at -0.370 and -0.290 V, respectively. Meanwhile, the catalytic activity of the protein toward the reduction of H2O2 was also studied, and a H2O2 biosensor was subsequently prepared. Its detection limit was 3.4 x 10(-7) M with a sensitivity of 0.0224 mu A per mu M of H2O2. The apparent Michaelis-Menten constant was calculated to be 107 mu M, which was lower than those of many horseradish peroxidase based H2O2 sensors, suggesting that structural rigidity of Hb could endow the protein a high peroxidase activity. (c) 2007 Elsevier B.V. All rights reserved.
Subject AreaChemistry ; Nuclear Science & Technology
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000248516100003
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7715
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorLi, GX (reprint author), Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
Recommended Citation
GB/T 7714
Wang, J,Liang, ZQ,Wang, LH,et al. Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2007,125(1):17.
APA Wang, J,Liang, ZQ,Wang, LH,Fan, CH,Li, GX,&Li, GX .(2007).Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin.SENSORS AND ACTUATORS B-CHEMICAL,125(1),17.
MLA Wang, J,et al."Electron transfer reactivity and catalytic activity of structurally rigidized hemoglobin".SENSORS AND ACTUATORS B-CHEMICAL 125.1(2007):17.
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