CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Enhanced specificity and efficiency of polymerase chain reactions using poly(amidoamine) dendrimers and derivatives
Cao, XY; Shi, XY; Yang, WC; Zhang, XD; Fan, CH(樊春海); Hu, J(胡钧); Shi, XY (reprint author), Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China
2009
Source PublicationANALYST
ISSN0003-2654
Volume134Issue:1Pages:87
AbstractThe polymerase chain reaction (PCR) has become a fundamental technique in molecular biology. Improvements to enhance the specificity and efficiency of PCR are always required and remain a great challenge. Here we introduce generation 4 (G4) and 5 (G5) poly(amidoamine) (PAMAM) dendrimers and their derivatives with different terminal groups as a novel class of enhancers to improve the specificity and efficiency of PCR. We show that the dendrimers are effective in optimizing error-prone two-round PCR and non-specific PCR in specificity and efficiency. With the increase of the number and density of terminal amine groups, the optimum concentration of polycationic dendrimers could be decreased to as low as 1.35 nM, which is four orders of magnitude smaller than that of fully acetylated dendrimers and carboxyl-terminated dendrimers. The present study underlines a fact that dendrimers could be used as a powerful additive to enhance the specificity and efficiency of PCR.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000261742600013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7718
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorShi, XY (reprint author), Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai 201620, Peoples R China
Recommended Citation
GB/T 7714
Cao, XY,Shi, XY,Yang, WC,et al. Enhanced specificity and efficiency of polymerase chain reactions using poly(amidoamine) dendrimers and derivatives[J]. ANALYST,2009,134(1):87.
APA Cao, XY.,Shi, XY.,Yang, WC.,Zhang, XD.,Fan, CH.,...&Shi, XY .(2009).Enhanced specificity and efficiency of polymerase chain reactions using poly(amidoamine) dendrimers and derivatives.ANALYST,134(1),87.
MLA Cao, XY,et al."Enhanced specificity and efficiency of polymerase chain reactions using poly(amidoamine) dendrimers and derivatives".ANALYST 134.1(2009):87.
Files in This Item:
File Name/Size DocType Version Access License
Enhanced specificity(558KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Cao, XY]'s Articles
[Shi, XY]'s Articles
[Yang, WC]'s Articles
Baidu academic
Similar articles in Baidu academic
[Cao, XY]'s Articles
[Shi, XY]'s Articles
[Yang, WC]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Cao, XY]'s Articles
[Shi, XY]'s Articles
[Yang, WC]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Enhanced specificity and efficiency of polymerase chain reactions using poly(amidoamine) dendrimers and derivatives.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.