Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface | |
| Wang, P; Li, H; Yang, HJ; Wang, HB（王化斌）; Lu, JH（吕军鸿）; Zhang, Y（张益）; Hu, J（胡钧）; Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| ISSN | 0927-7765 |
| Volume | 60Issue:2Pages:264 |
| Abstract | Preparation of distributed virus on a solid substrate is a prerequisite for investigation of the properties and individualism of virus, while many previous studies showed that virus has a tendency to aggregate on solid substrates. In this communication, we report a novel approach by which well-separated recombinant adeno-associated virus serotype 2 (rAAV2) could be prepared on bare mica surface. The key technique in this approach is the addition of less than 3% (v/v) glycerol into the virus solution and subsequently deposition onto mica surface for the sample preparation. The possible mechanisms are also briefly discussed. (C) 2007 Elsevier B.V. All rights reserved. |
| Subject Area | Nuclear Science & Technology ; Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7739 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, P,Li, H,Yang, HJ,et al. Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2007,60(2):264. |
| APA | Wang, P.,Li, H.,Yang, HJ.,Wang, HB.,Lu, JH.,...&Lu, JH .(2007).Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface.COLLOIDS AND SURFACES B-BIOINTERFACES,60(2),264. |
| MLA | Wang, P,et al."Glycerol facilitates the disaggregation of recombinant adeno-associated virus serotype 2 on mica surface".COLLOIDS AND SURFACES B-BIOINTERFACES 60.2(2007):264. |
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