CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine
Zhu, CF; Wen, YQ; Li, D(李迪); Wang, LH(王丽华); Song, SP(宋世平); Fan, CH(樊春海); Willner, I; Li, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2009
Source PublicationCHEMISTRY-A EUROPEAN JOURNAL
ISSN0947-6539
Volume15Issue:44Pages:11898
AbstractDNA replication plays a central role in living organisms. Unregulated or uncontrollable DNA replication is well known to result in many pathological states, such as cancer, autoimmune diseases, and viral/bacterial infections. We report that an aptamer-protein complex could indirectly inhibit in vitro replication of DNA. An isothermal DNA machine based on the strand-displacement amplification is employed to support our assumption. An antithrombin aptamer sequence is rationally encoded into the DNA replication template. Once thrombin binds to the template, the as-formed aptamer-protein complexes can, in turn, become a barrier to the polymerase and inhibit the DNA replication activities in both static and dynamic modes. The inhibition is successfully confirmed by both fluorescence and gel-electrophoresis experiments. Considering the availability of a broad library of aptamers and the existence of various DNA/protein interactions, our results imply the possibility for the rational regulation of DNA replication in vivo.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000272109100015
Citation statistics
Cited Times:66[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7753
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorLi, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhu, CF,Wen, YQ,Li, D,et al. Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(44):11898.
APA Zhu, CF.,Wen, YQ.,Li, D.,Wang, LH.,Song, SP.,...&Li, D .(2009).Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine.CHEMISTRY-A EUROPEAN JOURNAL,15(44),11898.
MLA Zhu, CF,et al."Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine".CHEMISTRY-A EUROPEAN JOURNAL 15.44(2009):11898.
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