Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine | |
| Zhu, CF; Wen, YQ; Li, D(李迪）; Wang, LH（王丽华）; Song, SP（宋世平）; Fan, CH（樊春海); Willner, I; Li, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2009 | |
| Source Publication | CHEMISTRY-A EUROPEAN JOURNAL
 |
| ISSN | 0947-6539 |
| Volume | 15Issue:44Pages:11898 |
| Abstract | DNA replication plays a central role in living organisms. Unregulated or uncontrollable DNA replication is well known to result in many pathological states, such as cancer, autoimmune diseases, and viral/bacterial infections. We report that an aptamer-protein complex could indirectly inhibit in vitro replication of DNA. An isothermal DNA machine based on the strand-displacement amplification is employed to support our assumption. An antithrombin aptamer sequence is rationally encoded into the DNA replication template. Once thrombin binds to the template, the as-formed aptamer-protein complexes can, in turn, become a barrier to the polymerase and inhibit the DNA replication activities in both static and dynamic modes. The inhibition is successfully confirmed by both fluorescence and gel-electrophoresis experiments. Considering the availability of a broad library of aptamers and the existence of various DNA/protein interactions, our results imply the possibility for the rational regulation of DNA replication in vivo. |
| Subject Area | Chemistry |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000272109100015 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7753 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Li, D (reprint author), Chinese Acad Sci, Phys Biol Lab, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, CF,Wen, YQ,Li, D,et al. Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine[J]. CHEMISTRY-A EUROPEAN JOURNAL,2009,15(44):11898. |
| APA | Zhu, CF.,Wen, YQ.,Li, D.,Wang, LH.,Song, SP.,...&Li, D .(2009).Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine.CHEMISTRY-A EUROPEAN JOURNAL,15(44),11898. |
| MLA | Zhu, CF,et al."Inhibition of the In Vitro Replication of DNA by an Aptamer-Protein Complex in an Autonomous DNA Machine".CHEMISTRY-A EUROPEAN JOURNAL 15.44(2009):11898. |
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