Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR | |
| Mi, LJ(米丽娟）; Zhu, HP; Zhang, XD; Hu, J（胡钧）; Fan, CH（樊春海）; Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | CHINESE SCIENCE BULLETIN
 |
| ISSN | 1001-6538 |
| Volume | 52Issue:17Pages:2345 |
| Abstract | Nanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification. |
| Subject Area | Polymer Science |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000249381100008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7777 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Mi, LJ,Zhu, HP,Zhang, XD,et al. Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR[J]. CHINESE SCIENCE BULLETIN,2007,52(17):2345. |
| APA | Mi, LJ,Zhu, HP,Zhang, XD,Hu, J,Fan, CH,&Hu, J .(2007).Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR.CHINESE SCIENCE BULLETIN,52(17),2345. |
| MLA | Mi, LJ,et al."Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR".CHINESE SCIENCE BULLETIN 52.17(2007):2345. |
| Files in This Item: | Download All | |||||
| File Name/Size | DocType | Version | Access | License | ||
| Mechanism of the int（1462KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment