CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR
Mi, LJ(米丽娟); Zhu, HP; Zhang, XD; Hu, J(胡钧); Fan, CH(樊春海); Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume52Issue:17Pages:2345
AbstractNanoparticle PCR is a novel method to optimize DNA amplification. It performs well in improving specificity, enhancing sensitivity and speed. Several mechanisms were proposed in previous studies: one was based on the interaction between gold nanoparticles (AuNPs) and DNA while the other was attributed to the heat transfer property of AuNPs. In this paper, we propose that the interaction between AuNPs and DNA polymerase can significantly influence PCR. First, the addition of DNA polymerase can eliminate the inhibitory effects of excess AuNPs. Second, the addition of AuNPs will increase yield of the desired PCR product and make the optimum concentration of DNA polymerase move to higher value. Third, while excess polymerase might inhibit amplification efficiency, AuNPs can reverse this process and the yield of PCR amplification. Based on these results we propose a possible mechanism that AuNPs might modulate the activity of polymerase and improve PCR amplification.
Subject AreaPolymer Science
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000249381100008
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7777
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Mi, LJ,Zhu, HP,Zhang, XD,et al. Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR[J]. CHINESE SCIENCE BULLETIN,2007,52(17):2345.
APA Mi, LJ,Zhu, HP,Zhang, XD,Hu, J,Fan, CH,&Hu, J .(2007).Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR.CHINESE SCIENCE BULLETIN,52(17),2345.
MLA Mi, LJ,et al."Mechanism of the interaction between Au nanoparticles and polymerase in nanoparticle PCR".CHINESE SCIENCE BULLETIN 52.17(2007):2345.
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