CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Nanogold-assisted multi-round polymerase to chain reaction (PCR)
Pan, JK; Li, HK; Cao, XY; Huang, JH; Zhang, XD; Fan, CH(樊春海); Hu, J(胡钧); Zhang, XD (reprint author), Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Nanobiol Lab, Bio X Life Sci Res Ctr, Shanghai 200240, Peoples R China
2007
Source PublicationJOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
ISSN1533-4880
Volume7Issue:12Pages:4428
AbstractWe have previously demonstrated that nanogold effectively enhances the specificity and yield of error-prone two-round polymerase chain reaction (PCR). Here we reported that, with the assistance of nanogold, we could perform multi-round PCR. In the presence of appropriate amount of 10 nm nanogold, we could obtain the target product even after six rounds of PCR, as manifested by a single bright band in gel electrophoresis (1% agarose). In fact, we could still observe the target band even at the 7th round of PCR, which nevertheless was accompanied by smearing bands (non-specific amplification). In contrast, in the absence of nanogold, the target band was completely lost only after four rounds of amplification. This marked difference in the performance of multi-round PCR clearly showed that nanogold was a powerful enhancer for PC,R. More importantly, with this nanogold-assisted multi-round PCR, it might be possible to produce a large amount of target DNA, or to amply very low copies of genomic DNA from rare sources.
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000252511300026
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7783
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorZhang, XD (reprint author), Shanghai Jiao Tong Univ, Coll Life Sci & Biotechnol, Nanobiol Lab, Bio X Life Sci Res Ctr, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Pan, JK,Li, HK,Cao, XY,et al. Nanogold-assisted multi-round polymerase to chain reaction (PCR)[J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2007,7(12):4428.
APA Pan, JK.,Li, HK.,Cao, XY.,Huang, JH.,Zhang, XD.,...&Zhang, XD .(2007).Nanogold-assisted multi-round polymerase to chain reaction (PCR).JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,7(12),4428.
MLA Pan, JK,et al."Nanogold-assisted multi-round polymerase to chain reaction (PCR)".JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY 7.12(2007):4428.
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