CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Optimization of specimen preparation of thin cell section for AFM observation
Li, XH; Ji, T; Hu, J(胡钧); Sun, JL; Sun, JL (reprint author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Nanobiol Lab, BioX Life Sci Res Ctr, 800 Dongchuan Rd, Shanghai 200240, Peoples R China
2008
Source PublicationULTRAMICROSCOPY
ISSN0304-3991
Volume108Issue:9Pages:826
AbstractHigh resolution imaging of intracellular structures of ultrathin cell section samples is critical to the performance of precise manipulation by atomic force microscopy (A FM). Here, we test the effect of Multiple factors during section sample preparation on the quality of the AFM image. These factors include the embedding materials. the annealing process of the specimen block,section thickness. and section side. We found that neither the embedding materials nor the temperature and speed of the annealing process has any effect on AFM image resolution. However, the section thickness and section side significantly affect the surface topography and AFM image resolution. By systematically testing the image quality of both sides of cell sections over a wide range of thickness (40-1000 nm). we found that the best resolution was obtained with upper-side sections approximately 50-100nm thick. With these samples, we Could observe Precise Structure details of the cell, including its membrane, nucleoli. and other organelles. Similar results were obtained for other cell types. including Tca8113, C6. and ECV-304. In brief, by optimizingg the condition o ultrathin cell section preparation. we were able to obtain high resolution intracellular AFM images, which provide an essential basis for further AFM manipulation. (c) 2008 Elsevier B.V. All rights reserved.
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000258747600004
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7790
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorSun, JL (reprint author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Nanobiol Lab, BioX Life Sci Res Ctr, 800 Dongchuan Rd, Shanghai 200240, Peoples R China
Recommended Citation
GB/T 7714
Li, XH,Ji, T,Hu, J,et al. Optimization of specimen preparation of thin cell section for AFM observation[J]. ULTRAMICROSCOPY,2008,108(9):826.
APA Li, XH,Ji, T,Hu, J,Sun, JL,&Sun, JL .(2008).Optimization of specimen preparation of thin cell section for AFM observation.ULTRAMICROSCOPY,108(9),826.
MLA Li, XH,et al."Optimization of specimen preparation of thin cell section for AFM observation".ULTRAMICROSCOPY 108.9(2008):826.
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