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| Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis | |
| Zhang, ZX; Hao, SM; Zhu, HP; Wang, WF(王文峰）; Wang, WF (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2008 | |
| Source Publication | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
 |
| ISSN | 1011-1344 |
| Volume | 92Issue:2Pages:77 |
| Abstract | Photoprocesses of 1,4-naphthoquinone (NQ) and its photoreactions with lysozyme in acetonitrile/water (3: 1, v/v) solution were studied using 355 nm laser flash photolysis technique combined with electrophoresis and turbidimetric assay. The transient spectra of NQ were observed and the transient species were assigned. The electron transfer process from N,N,N ',N '-tetramethyl-p-phenylenediamine (TMPD) to NQ triplet state ((3)NQ(center dot)) was investigated and the rate constant was determined to be k(t1) = 2.0 x 10(10) M-1 s(-1). it has been found that (3)NQ(center dot) can abstract hydrogen atom from lysozyme with a rate constant of k(t2) = 2.4 x 10(10) M-1 s(-1). Furthermore, the results of steady-state analysis suggested that lysozyme can be damaged by NQ irradiated with UVA light influenced by the concentration of NQ and the gas saturated in the solution. The mechanisms of photosensitized damage of lysozyme were discussed. (c) 2008 Elsevier B.V. All rights reserved. |
| Subject Area | Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000258906600002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7801 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Wang, WF (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, ZX,Hao, SM,Zhu, HP,et al. Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,2008,92(2):77. |
| APA | Zhang, ZX,Hao, SM,Zhu, HP,Wang, WF,&Wang, WF .(2008).Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY,92(2),77. |
| MLA | Zhang, ZX,et al."Photoreactions of 1,4-naphthoquinone with lysozyme studied by laser flash photolysis and steady-state analysis".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 92.2(2008):77. |
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