Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Quantitative analysis of nucleotide mixtures with terahertz time domain spectroscopy | |
| Zhang, ZY(张增艳）; Xiao, TQ（肖体乔）; Zhao, HW（赵红卫）; Yu, XH（余笑寒）; Xi, ZJ（席再军）; Xu, HJ（徐洪杰）; Xiao, TQ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2008 | |
| Source Publication | SPECTROSCOPY AND SPECTRAL ANALYSIS
 |
| ISSN | 1000-0593 |
| Volume | 28Issue:9Pages:1990 |
| Abstract | Adenosine, thymidine, guanosine, cytidine and uridine form the building blocks of ribose nucleic acid (RNA) and deoxyribose nucleic acid (DNA). Nucleosides and their derivants are all have biological activities. Some of them can be used as medicine directly or as materials to synthesize other medicines. It is meaningful to detect the component and content in nucleosides mixtures. In the present paper, components and contents of the mixtures of adenosine, thymidine, guanosine, cytidine and uridine were analyzed. THz absorption spectra of pure nucleosides were set as standard spectra. The mixture's absorption Spectra were analyzed by linear regression with non-negative constraint to identify the components and their relative content in the mixtures. The experimental and analyzing results show that it is simple and effective to get the components and their relative percentage in the mixtures by terahertz time domain spectroscopy with a relative error less than 10%. Component which is absent could be excluded exactly by this method, and the error sources were also analyzed. All the experiments and analysis confirms that this method is of no damage or contamination to the sample. This means that it will be a simple, effective and new method in biochemical materials analysis, which extends the application field of THz-TDS. |
| Subject Area | Engineering ; Environmental Sciences & Ecology |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000259688600009 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7808 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Xiao, TQ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, ZY,Xiao, TQ,Zhao, HW,et al. Quantitative analysis of nucleotide mixtures with terahertz time domain spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2008,28(9):1990. |
| APA | Zhang, ZY.,Xiao, TQ.,Zhao, HW.,Yu, XH.,Xi, ZJ.,...&Xiao, TQ .(2008).Quantitative analysis of nucleotide mixtures with terahertz time domain spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,28(9),1990. |
| MLA | Zhang, ZY,et al."Quantitative analysis of nucleotide mixtures with terahertz time domain spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 28.9(2008):1990. |
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