Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Study on the interaction of colloidal gold with Taq DNA polymerase | |
| Zhu, HP(朱红平）; Mi, LJ（米丽娟）; Chen, SM（陈仕谋）; Wang, WF（王文锋）; Yao, SD（姚思德）; Zhu, HP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | CHINESE JOURNAL OF CHEMISTRY
 |
| ISSN | 1001-604X |
| Volume | 25Issue:9Pages:1233 |
| Abstract | The interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold. |
| Subject Area | Chemistry |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000249473600003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7842 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Zhu, HP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zhu, HP,Mi, LJ,Chen, SM,et al. Study on the interaction of colloidal gold with Taq DNA polymerase[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(9):1233. |
| APA | Zhu, HP,Mi, LJ,Chen, SM,Wang, WF,Yao, SD,&Zhu, HP .(2007).Study on the interaction of colloidal gold with Taq DNA polymerase.CHINESE JOURNAL OF CHEMISTRY,25(9),1233. |
| MLA | Zhu, HP,et al."Study on the interaction of colloidal gold with Taq DNA polymerase".CHINESE JOURNAL OF CHEMISTRY 25.9(2007):1233. |
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