CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Study on the interaction of colloidal gold with Taq DNA polymerase
Zhu, HP(朱红平); Mi, LJ(米丽娟); Chen, SM(陈仕谋); Wang, WF(王文锋); Yao, SD(姚思德); Zhu, HP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationCHINESE JOURNAL OF CHEMISTRY
ISSN1001-604X
Volume25Issue:9Pages:1233
AbstractThe interaction of colloidal gold with Taq DNA polymerase (Taq) was investigated in this study. Taq-gold conjugate was formed by adding the enzyme to the colloidal gold solution, as evidenced by UV-Vis spectroscopy, X-ray photoelectron spectroscopy, and photon cross correlation spectroscopy measurements. The conjugate was further characterized by transmission electron microscopy. It was found that the Taq-gold conjugate particles were still spherical and well-dispersed. The influence of gold nanoparticles on the bioactivity of Taq was studied by analyzing the yield of the polymerase chain reaction amplification. Results indicated that the enzymatic activity of Taq decreased after interaction with the colloidal gold.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000249473600003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7842
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorZhu, HP (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhu, HP,Mi, LJ,Chen, SM,et al. Study on the interaction of colloidal gold with Taq DNA polymerase[J]. CHINESE JOURNAL OF CHEMISTRY,2007,25(9):1233.
APA Zhu, HP,Mi, LJ,Chen, SM,Wang, WF,Yao, SD,&Zhu, HP .(2007).Study on the interaction of colloidal gold with Taq DNA polymerase.CHINESE JOURNAL OF CHEMISTRY,25(9),1233.
MLA Zhu, HP,et al."Study on the interaction of colloidal gold with Taq DNA polymerase".CHINESE JOURNAL OF CHEMISTRY 25.9(2007):1233.
Files in This Item: Download All
File Name/Size DocType Version Access License
Study on the interac(87KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, HP(朱红平)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Chen, SM(陈仕谋)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, HP(朱红平)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Chen, SM(陈仕谋)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, HP(朱红平)]'s Articles
[Mi, LJ(米丽娟)]'s Articles
[Chen, SM(陈仕谋)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Study on the interaction of colloidal gold with Taq DNA polymerase.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.