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Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability | |
Lv, M; Zhu, EG(朱恩高); Su, YY; Li, QN(李晴暖); Li, WX(李文新); Zhao, Y(赵云); Huang, Q(黄庆); Huang, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
2009 | |
Source Publication | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
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ISSN | 1082-6068 |
Volume | 39Issue:4Pages:429 |
Abstract | Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability. |
Subject Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
Indexed By | SCI |
Language | 英语 |
Funding Project | 应物所项目组 |
WOS ID | WOS:000269668300008 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.sinap.ac.cn/handle/331007/7868 |
Collection | 中科院上海应用物理研究所2004-2010年 |
Corresponding Author | Huang, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
Recommended Citation GB/T 7714 | Lv, M,Zhu, EG,Su, YY,et al. Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2009,39(4):429. |
APA | Lv, M.,Zhu, EG.,Su, YY.,Li, QN.,Li, WX.,...&Huang, Q .(2009).Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,39(4),429. |
MLA | Lv, M,et al."Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 39.4(2009):429. |
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