Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability | |
| Lv, M; Zhu, EG(朱恩高）; Su, YY; Li, QN（李晴暖）; Li, WX（李文新）; Zhao, Y（赵云）; Huang, Q（黄庆）; Huang, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2009 | |
| Source Publication | PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
 |
| ISSN | 1082-6068 |
| Volume | 39Issue:4Pages:429 |
| Abstract | Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability. |
| Subject Area | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000269668300008 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/7868 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Huang, Q (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Lv, M,Zhu, EG,Su, YY,et al. Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2009,39(4):429. |
| APA | Lv, M.,Zhu, EG.,Su, YY.,Li, QN.,Li, WX.,...&Huang, Q .(2009).Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,39(4),429. |
| MLA | Lv, M,et al."Trypsin-Gold Nanoparticle Conjugates: Binding, Enzymatic Activity, and Stability".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 39.4(2009):429. |
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