CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
A centrifugation-based method for preparation of gold nanoparticles and its application in biodetection
Alternative Titlemannual of IR
Liang, ZQ; Zhang, J; Wang, LH(王丽华); Song, SP(宋世平); Fan, CH(樊春海); Li, GX; Wang, LH (reprint author), Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
2007
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
Volume8Issue:6Pages:526
AbstractGold nanoparticles (AuNPs) have found widespread applications in life sciences. While synthesis of monodispersed AuNPs has been fairly convenient by using chemical reduction of chloroauric acid by sodium citrate, we found that AuNPs of high quality and high concentrations were not readily obtained via this method. As an example, we showed that monodispersed 13-nm AuNPs were readily synthesized at relatively low concentrations ( e. g. 3.5 nM); in contrast, 13-nm AuNPs of 17 nM obtained by the direct reduction method were irregularly shaped and not well dispersed. In this work, we demonstrated that AuNPs of high concentration could be prepared by a two-step approach, i.e. chemical reduction at low concentrations and subsequent centrifugation. Compared to the direct reduction method, this new two-step method led to AuNPs with high salt resistance and high stability, which are essential for the preparation of DNA-AuNPs conjugates for DNA biodetection.
KeywordGold Nanoparticles Stability Centrifugation Dna Self-assembly
Subject AreaPhysics
Indexed Bysci
Language英语
Funding Project应物所项目组
WOS IDWOS:000247597900006
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/7998
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorWang, LH (reprint author), Nanjing Univ, Dept Biochem, Nanjing 210093, Peoples R China
Recommended Citation
GB/T 7714
Liang, ZQ,Zhang, J,Wang, LH,et al. A centrifugation-based method for preparation of gold nanoparticles and its application in biodetection[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2007,8(6):526.
APA Liang, ZQ.,Zhang, J.,Wang, LH.,Song, SP.,Fan, CH.,...&Wang, LH .(2007).A centrifugation-based method for preparation of gold nanoparticles and its application in biodetection.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,8(6),526.
MLA Liang, ZQ,et al."A centrifugation-based method for preparation of gold nanoparticles and its application in biodetection".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 8.6(2007):526.
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