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| A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP | |
| Zuo, XL; Song, SP（宋世平）; Zhang, J; Pan, D; Wang, LH（王丽华）; Fan, CH（樊春海）; Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| ISSN | 0002-7863 |
| Volume | 129Issue:5Pages:1042 |
| Abstract | In this communication, we report a highly generalizable strategy, target-responsive electrochemical aptamer switch (TREAS) for the development of aptamer-based biosensors. In a typical TREAS design for ATP detection, the aptamer oligonucleotide dually labeled with thiol and ferrocene groups is hybridized with its complementary strand, and the thiolated duplex is self-assembled on a gold electrode. This duplex is responsive to the target ATP, which liberates the complementary strand and forms the aptamer-target complex. The electroactive ferrocene moiety, which is distal to the electrode surface in the absence of ATP, is moved to the proximal position during the binding-induced structural transition. This binding turns on the electron transfer and leads to measurable electrochemical signals for quantification of ATP. We then demonstrate that TREAS is a signal-on, reagentless sensor that can selectively detect ATP and features both generalizability and simplicity in design. |
| Subject Area | Chemistry ; Physics |
| Indexed By | sci |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000243840100015 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8006 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zuo, XL,Song, SP,Zhang, J,et al. A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2007,129(5):1042. |
| APA | Zuo, XL.,Song, SP.,Zhang, J.,Pan, D.,Wang, LH.,...&Fan, CH .(2007).A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,129(5),1042. |
| MLA | Zuo, XL,et al."A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129.5(2007):1042. |
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