CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP
Zuo, XL; Song, SP(宋世平); Zhang, J; Pan, D; Wang, LH(王丽华); Fan, CH(樊春海); Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume129Issue:5Pages:1042
AbstractIn this communication, we report a highly generalizable strategy, target-responsive electrochemical aptamer switch (TREAS) for the development of aptamer-based biosensors. In a typical TREAS design for ATP detection, the aptamer oligonucleotide dually labeled with thiol and ferrocene groups is hybridized with its complementary strand, and the thiolated duplex is self-assembled on a gold electrode. This duplex is responsive to the target ATP, which liberates the complementary strand and forms the aptamer-target complex. The electroactive ferrocene moiety, which is distal to the electrode surface in the absence of ATP, is moved to the proximal position during the binding-induced structural transition. This binding turns on the electron transfer and leads to measurable electrochemical signals for quantification of ATP. We then demonstrate that TREAS is a signal-on, reagentless sensor that can selectively detect ATP and features both generalizability and simplicity in design.
Subject AreaChemistry ; Physics
Indexed Bysci
Language英语
Funding Project应物所项目组
WOS IDWOS:000243840100015
Citation statistics
Cited Times:452[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8006
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorFan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zuo, XL,Song, SP,Zhang, J,et al. A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2007,129(5):1042.
APA Zuo, XL.,Song, SP.,Zhang, J.,Pan, D.,Wang, LH.,...&Fan, CH .(2007).A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,129(5),1042.
MLA Zuo, XL,et al."A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129.5(2007):1042.
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