CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles
Hong, J(洪军); Gong, PJ(龚培军); Yu, JH; Xu, DM(徐冬梅); Sun, HW(孙汉文); Yao, S(姚思德); Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2006
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
Volume42Issue:40606Pages:99
AbstractMagnetic nanoparticles, covered by a polymeric hydrophilic nanolayer containing reactive amino groups, were obtained via Hoffman degradation of the polyacrylamide-coated Fe3O4 nanoparticles synthesized by photochemical in situ polymerization, and then conjugated the model enzyme-alpha-chymotrypsin (CT) by use of EDC. HCl and NHS at room temperatures. The mechanism of photochemical in situ polymerization was briefly proposed in this paper. Superparamagnetic properties were retained for Fe3O4 after enzyme immobilization while slightly reducing the value of saturation magnetization. Crystalline structure of Fe3O4 after CT immobilization was consistent with that of the freshly prepared Fe3O4 by X-ray diffraction (XRD) analysis. The binding capacity was 69 and 61 mg enzyme/g nanogel determined by thermogravimetric (TG) analysis and by standard BCA protein assay, respectively. Specific activity of the immobilized CT was 0.93 U/(mg min), only 59.3% as that of free CT. Thermal stability of CT was improved after being bound to the amine-functionalized magnetic nanogel. (c) 2006 Elsevier B.V. All rights reserved.
KeywordImmobilized Enzyme Photochemical In Situ Polymerization Alpha-chymotrypsin Magnetic Nanogel
Subject AreaChemistry
Indexed Bysci
Language英语
Funding Project应物所项目组
WOS IDWOS:000241660700006
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8025
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorYao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Hong, J,Gong, PJ,Yu, JH,et al. Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2006,42(40606):99.
APA Hong, J.,Gong, PJ.,Yu, JH.,Xu, DM.,Sun, HW.,...&Yao, SD .(2006).Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,42(40606),99.
MLA Hong, J,et al."Conjugation of alpha-chymotrypsin on a polymeric hydrophilic nanolayer covering magnetic nanoparticles".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 42.40606(2006):99.
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