Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Conjugation of enzyme on superparamagnetic nanogels covered with carboxyl groups | |
| Hong, J（洪军）; Xu, DM（徐冬梅）; Gong, PJ（龚培军）; Ma, HJ（马红娟）; Dong, L; Yao, SD（姚思德）; Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| ISSN | 1570-0232 |
| Volume | 850Issue:40545Pages:499 |
| Abstract | alpha-Chymotrypsin (CT) as model enzyme was conjugated onto the novel carboxyl-functionalized superparamagnetic nanogels, prepared via facile photochemical in situ polymerization, by using 1-ethyl-3-(3-dimethylaminepropyl) carbodiimide (EDC) as coupling reagent. The obtained magnetic immobilized enzyme was characterized by use of photo correlation spectroscopy (PCS), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) measurement, thermogravimetric (TG) analysis and vibrating sample magnetometer (VSM) measurement. PCS result showed that the immobilized enzyme was 68 nm in diameter while the magnetic nanogels with carboxyl groups were only 38 nm; enzyme immobilization led to pronounced change in size. Superparamagnetic properties were retained for Fe3O4 after enzyme immobilization while slightly reducing its value of saturation magnetization. Immobilization and surface coating did not induce phase change of Fe3O4 by XRD analysis. The binding capacity was 30 mg enzyme/g and 37.5 mg enzyme/g nanogel determined by TG analysis and BCA protein assay, respectively. Specific activity of the immobilized CT was calculated to be 0.77 U/(mg min), 82.7% as that of the free form. (c) 2007 Elsevier B.V. All rights reserved. |
| Keyword | Carboxyl-functionalized Magnetic Nanogels Immobilized Enzyme Magnetic Separation Photochemical In Situ Polymerization |
| Subject Area | Engineering |
| Indexed By | sci |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000246703500064 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8026 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Hong, J,Xu, DM,Gong, PJ,et al. Conjugation of enzyme on superparamagnetic nanogels covered with carboxyl groups[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,850(40545):499. |
| APA | Hong, J.,Xu, DM.,Gong, PJ.,Ma, HJ.,Dong, L.,...&Yao, SD .(2007).Conjugation of enzyme on superparamagnetic nanogels covered with carboxyl groups.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,850(40545),499. |
| MLA | Hong, J,et al."Conjugation of enzyme on superparamagnetic nanogels covered with carboxyl groups".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 850.40545(2007):499. |
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