CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Covalent binding of alpha-chymotrypsin on the magnetic nanogels covered by amino groups
Hong, J(洪军); Xu, DM(徐冬梅); Gong, PJ(龚培军); Sun, HW(孙汉文); Dong, L; Yao, SD(姚思德); Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationJOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN1381-1177
Volume45Issue:40606Pages:84
AbstractA new aminated carrier-magnetic nanogels covered by amino groups, was obtained by Hoffman degradation of polyacrylamide-coated Fe3O4 nanoparticles prepared by photochemical polymerization. alpha-Chymotrypsin (CT) was covalently bound to the magnetic nanogels by use of 1-ethyl3-(3-dimethylaminepropyl) carbodiimide and N-hydroxysuccinimide at room temperature. Immobilization time, pH value of the reaction mixture and proportion of CT to the magnetic nanogels were investigated to obtain the optimum condition for CT immobilization. The maximal specific activity of the bound CT was determined to be 0.93 U/(mg min), 59.3% of free counterpart. The maximal binding capacity was measured to be 102 mg enzyme/g nanogel. Furthermore, the bound CT exhibited good thermal stability, storage stability and reusability. (c) 2007 Elsevier B.V. All rights reserved.
KeywordMagnetic Nanogels Immobilized Enzyme Photochemical Polymerization Alpha-chymotrypsin
Subject AreaPhysics
Indexed Bysci
Language英语
Funding Project应物所项目组
WOS IDWOS:000245350900003
Citation statistics
Cited Times:50[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8028
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorYao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Hong, J,Xu, DM,Gong, PJ,et al. Covalent binding of alpha-chymotrypsin on the magnetic nanogels covered by amino groups[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2007,45(40606):84.
APA Hong, J.,Xu, DM.,Gong, PJ.,Sun, HW.,Dong, L.,...&Yao, SD .(2007).Covalent binding of alpha-chymotrypsin on the magnetic nanogels covered by amino groups.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,45(40606),84.
MLA Hong, J,et al."Covalent binding of alpha-chymotrypsin on the magnetic nanogels covered by amino groups".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 45.40606(2007):84.
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