Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Electrochemical interrogation of DNA monolayers on gold surfaces | |
| Lao, RJ; Song, SP（宋世平）; Wu, HP; Wang, LH（王丽华）; Zhang, ZZ; He, L; Fan, CH（樊春海）; Zhang, ZZ (reprint author), Shanghai Jiao Tong Univ, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China | |
| 2005 | |
| Source Publication | ANALYTICAL CHEMISTRY
 |
| ISSN | 0003-2700 |
| Volume | 77Issue:19Pages:6475 |
| Abstract | In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chrono-coulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au-DNA interactions existed even with the treatment of mercaptobexanol, which was known to competitively remove loosely bound. DNA at gold surfaces. While both thiolated and nonthiolated DNA formed monolayers on gold surfaces, their hybridization abilities were distinctly different. In contrast to thiolated DNA probes, nonthiolated DNA probes immobilized at gold surfaces were essentially nonhybridizable. The experimental results presented here might be useful for the design of high-performance electrochemical DNA sensors. |
| Subject Area | Physics |
| Indexed By | sci |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000232443700061 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8049 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Zhang, ZZ (reprint author), Shanghai Jiao Tong Univ, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China |
| Recommended Citation GB/T 7714 | Lao, RJ,Song, SP,Wu, HP,et al. Electrochemical interrogation of DNA monolayers on gold surfaces[J]. ANALYTICAL CHEMISTRY,2005,77(19):6475. |
| APA | Lao, RJ.,Song, SP.,Wu, HP.,Wang, LH.,Zhang, ZZ.,...&Zhang, ZZ .(2005).Electrochemical interrogation of DNA monolayers on gold surfaces.ANALYTICAL CHEMISTRY,77(19),6475. |
| MLA | Lao, RJ,et al."Electrochemical interrogation of DNA monolayers on gold surfaces".ANALYTICAL CHEMISTRY 77.19(2005):6475. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Electrochemical Inte（168KB） | 开放获取 | -- | View Download | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment