Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Height measurement of DNA molecules with lift mode AFM | |
| Guo, YC; Zhou, XF（周星飞）; Sun, JL; Li, MQ（李铭乾）; Hu, J（胡钧）; Sun, JL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2004 | |
| Source Publication | CHINESE SCIENCE BULLETIN
 |
| ISSN | 1001-6538 |
| Volume | 49Issue:15Pages:1574 |
| Abstract | The height of double-stranded DNA (dsDNA) is measured by lift mode AFM combined with conventional tapping mode AFM. While the tip scan height is raised step by step, the tip pressure on sample is decreased gradually. As a result, the deformation of the DNA strands decreases, and the height of double-stranded DNA (dsDNA) molecule can be deduced by the tip lift height. The measured height of dsDNA is 1.5 +/- 0.2 nm in lift mode, but only 0.8 +/- 0.2 nm in conventional tapping mode. This demonstrates that the tip pressure is a key factor in soft sample height measurement resulting in artificating lower values via conventional tapping mode. |
| Keyword | Lift Mode Afm Tapping Mode Afm Lift Scan Height Elastic Deformation Height Measurement |
| Subject Area | Physics |
| Indexed By | sci |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000224314400004 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8071 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Sun, JL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Guo, YC,Zhou, XF,Sun, JL,et al. Height measurement of DNA molecules with lift mode AFM[J]. CHINESE SCIENCE BULLETIN,2004,49(15):1574. |
| APA | Guo, YC,Zhou, XF,Sun, JL,Li, MQ,Hu, J,&Sun, JL .(2004).Height measurement of DNA molecules with lift mode AFM.CHINESE SCIENCE BULLETIN,49(15),1574. |
| MLA | Guo, YC,et al."Height measurement of DNA molecules with lift mode AFM".CHINESE SCIENCE BULLETIN 49.15(2004):1574. |
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