CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Height measurement of DNA molecules with lift mode AFM
Guo, YC; Zhou, XF(周星飞); Sun, JL; Li, MQ(李铭乾); Hu, J(胡钧); Sun, JL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2004
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume49Issue:15Pages:1574
AbstractThe height of double-stranded DNA (dsDNA) is measured by lift mode AFM combined with conventional tapping mode AFM. While the tip scan height is raised step by step, the tip pressure on sample is decreased gradually. As a result, the deformation of the DNA strands decreases, and the height of double-stranded DNA (dsDNA) molecule can be deduced by the tip lift height. The measured height of dsDNA is 1.5 +/- 0.2 nm in lift mode, but only 0.8 +/- 0.2 nm in conventional tapping mode. This demonstrates that the tip pressure is a key factor in soft sample height measurement resulting in artificating lower values via conventional tapping mode.
KeywordLift Mode Afm Tapping Mode Afm Lift Scan Height Elastic Deformation Height Measurement
Subject AreaPhysics
Indexed Bysci
Language英语
Funding Project应物所项目组
WOS IDWOS:000224314400004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8071
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorSun, JL (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Guo, YC,Zhou, XF,Sun, JL,et al. Height measurement of DNA molecules with lift mode AFM[J]. CHINESE SCIENCE BULLETIN,2004,49(15):1574.
APA Guo, YC,Zhou, XF,Sun, JL,Li, MQ,Hu, J,&Sun, JL .(2004).Height measurement of DNA molecules with lift mode AFM.CHINESE SCIENCE BULLETIN,49(15),1574.
MLA Guo, YC,et al."Height measurement of DNA molecules with lift mode AFM".CHINESE SCIENCE BULLETIN 49.15(2004):1574.
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