Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation | |
| Li, B（李宾）; Zhang, Y（张益）; Yan, SH; Lu, JH; Ye, M（叶鸣）; Li, MQ（李明乾）; Hu, J（胡钧）; Zhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| ISSN | 0002-7863 |
| Volume | 129Issue:21Pages:6668 |
| Abstract | Controlling chemical and biochemical reactions on nanoscale at the single-molecule level is of great interest due to its possibility for exploring new phenomena and/or unraveling novel mechanisms that are inaccessible for traditional bulk systems. In this paper, we report how DNase I, a well-known and widely used nonspecific endonuclease, digested DNA at controlled sites by using an approach termed combined-dynamic dip-pen nanolithography (CDDPN). High efficient digestion has been realized even under an ambient condition on the surfaces. We attribute this phenomenon to a high effective concentration of DNase I when confined in the nanospots. |
| Subject Area | Chemistry ; Nuclear Science & Technology |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000246686700003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8162 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Zhang, Y (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Li, B,Zhang, Y,Yan, SH,et al. Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2007,129(21):6668. |
| APA | Li, B.,Zhang, Y.,Yan, SH.,Lu, JH.,Ye, M.,...&Zhang, Y .(2007).Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,129(21),6668. |
| MLA | Li, B,et al."Positioning scission of single DNA molecules with nonspecific endonuclease based on nanomanipulation".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 129.21(2007):6668. |
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