Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Secondary structural formation of alpha-synuclein amyloids as revealed by g-factor of solid-state circular dichroism | |
| Lin, XJ; Zhang, F; Xie, YY; Bao, WJ; He, JH（何建华）; Hu, HY; Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China | |
| 2006 | |
| Source Publication | BIOPOLYMERS
 |
| ISSN | 0006-3525 |
| Volume | 83Issue:3Pages:226 |
| Abstract | alpha-Synuclein (alpha-Syn) has been identified as a component of intracellular fibrillar deposits in Parkinson's disease. Though the real pathogenesis is still unknown, many investigations have revealed that conformational alteration and fibril formation of alpha-Syn protein have an important role in causing the disease. In this work, we introduced the g-factor spectra of solid-state circular dichroism to estimate the secondary structure contents of alpha-Syn fragments in amyloids. Fourier-transform infrared (FTIR) was also applied to confirm the structural formation. The results suggest that the central hydrophobic region is critical for beta-sheet formation and the conformational alteration is the foundation of protein abnormal aggregation. The research provides a practical approach to estimate the secondary structure contents of protein amyloids and further insight into the relevance of structural transformation and amyloidogenesis. (c) 2006 Wiley Periodicals, Inc. |
| Keyword | Alpha-synuclein Circular Dichroism G-factor Aggregation Gav Motif Ftir |
| Subject Area | Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000240709800003 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8187 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Hu, HY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China |
| Recommended Citation GB/T 7714 | Lin, XJ,Zhang, F,Xie, YY,et al. Secondary structural formation of alpha-synuclein amyloids as revealed by g-factor of solid-state circular dichroism[J]. BIOPOLYMERS,2006,83(3):226. |
| APA | Lin, XJ.,Zhang, F.,Xie, YY.,Bao, WJ.,He, JH.,...&Hu, HY .(2006).Secondary structural formation of alpha-synuclein amyloids as revealed by g-factor of solid-state circular dichroism.BIOPOLYMERS,83(3),226. |
| MLA | Lin, XJ,et al."Secondary structural formation of alpha-synuclein amyloids as revealed by g-factor of solid-state circular dichroism".BIOPOLYMERS 83.3(2006):226. |
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