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Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): Effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes
Zhang, J(张炯); Song, SP(宋世平); Zhang, LY(张兰勇); Wang, LH(王丽华); Wu, HP; Pan, D; Fan, CH(樊春海); Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2006
Source PublicationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY
ISSN0002-7863
Volume128Issue:26Pages:8575
AbstractWe herein report a novel nanoparticle-based electrochemical DNA detection approach. This DNA sensor is based on a "sandwich" detection strategy, which involves capture probe DNA immobilized on gold electrodes and reporter probe DNA labeled with gold nanoparticles that flank the target DNA sequence. Electrochemical signals are generated by chronocoulometric interrogation of [Ru(NH(3))(6)](3+) that quantitatively binds to surface-confined capture probe DNA via electrostatic interactions. We demonstrated that the incorporation of a gold nanoparticle in this sensor design significantly enhanced the sensitivity and the selectivity. Nanoscale control of the self-assembly process of DNA probes at gold electrodes further increased the sensor performance. As a result of these two combined effects, this DNA sensor could detect as low as femtomolar (zeptomoles) DNA targets and exhibited excellent selectivity against even a single-base mismatch. In addition, this novel DNA sensor showed fairly good reproducibility, stability, and reusability.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000238590000045
Citation statistics
Cited Times:380[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8188
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorFan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, J,Song, SP,Zhang, LY,et al. Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): Effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2006,128(26):8575.
APA Zhang, J.,Song, SP.,Zhang, LY.,Wang, LH.,Wu, HP.,...&Fan, CH .(2006).Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): Effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,128(26),8575.
MLA Zhang, J,et al."Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): Effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 128.26(2006):8575.
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