CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation
An, HJ; Huang, JH; Lu, M; Li, XL; Lu, JH; Li, HK; Zhang, Y(张益); Li, MQ(李铭乾); Hu, J(胡钧); Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
2007
Source PublicationNANOTECHNOLOGY
ISSN0957-4484
Volume18Issue:22
AbstractWe show new approaches towards a novel single-molecule sequencing strategy which consists of high-resolution positioning isolation of overlapping DNA fragments with atomic force microscopy (AFM), subsequent single-molecule PCR amplification and conventional Sanger sequencing. In this study, a DNA labelling technique was used to guarantee the accuracy in positioning the target DNA. Single-molecule multiplex PCR was carried out to test the contamination. The results showed that the two overlapping DNA fragments isolated by AFM could be successfully sequenced with high quality and perfect contiguity, indicating that single-base resolution and long-coverage sequencing have been achieved simultaneously.
Subject AreaInstruments & Instrumentation ; Nuclear Science & Technology ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000246590400001
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8190
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorLu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
An, HJ,Huang, JH,Lu, M,et al. Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation[J]. NANOTECHNOLOGY,2007,18(22).
APA An, HJ.,Huang, JH.,Lu, M.,Li, XL.,Lu, JH.,...&Lu, JH .(2007).Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation.NANOTECHNOLOGY,18(22).
MLA An, HJ,et al."Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation".NANOTECHNOLOGY 18.22(2007).
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