Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation | |
| An, HJ; Huang, JH; Lu, M; Li, XL; Lu, JH; Li, HK; Zhang, Y（张益）; Li, MQ（李铭乾）; Hu, J（胡钧）; Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | NANOTECHNOLOGY
 |
| ISSN | 0957-4484 |
| Volume | 18Issue:22 |
| Abstract | We show new approaches towards a novel single-molecule sequencing strategy which consists of high-resolution positioning isolation of overlapping DNA fragments with atomic force microscopy (AFM), subsequent single-molecule PCR amplification and conventional Sanger sequencing. In this study, a DNA labelling technique was used to guarantee the accuracy in positioning the target DNA. Single-molecule multiplex PCR was carried out to test the contamination. The results showed that the two overlapping DNA fragments isolated by AFM could be successfully sequenced with high quality and perfect contiguity, indicating that single-base resolution and long-coverage sequencing have been achieved simultaneously. |
| Subject Area | Instruments & Instrumentation ; Nuclear Science & Technology ; Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000246590400001 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8190 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | An, HJ,Huang, JH,Lu, M,et al. Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation[J]. NANOTECHNOLOGY,2007,18(22). |
| APA | An, HJ.,Huang, JH.,Lu, M.,Li, XL.,Lu, JH.,...&Lu, JH .(2007).Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation.NANOTECHNOLOGY,18(22). |
| MLA | An, HJ,et al."Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation".NANOTECHNOLOGY 18.22(2007). |
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