Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation

CAS OpenIR > 中科院上海应用物理研究所2004-2010年

	Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation
	An, HJ; Huang, JH; Lu, M; Li, XL; Lu, JH; Li, HK; Zhang, Y（张益）; Li, MQ（李铭乾）; Hu, J（胡钧）; Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
	2007
Source Publication	NANOTECHNOLOGY
ISSN	0957-4484
Volume	18 Issue:22
Abstract	We show new approaches towards a novel single-molecule sequencing strategy which consists of high-resolution positioning isolation of overlapping DNA fragments with atomic force microscopy (AFM), subsequent single-molecule PCR amplification and conventional Sanger sequencing. In this study, a DNA labelling technique was used to guarantee the accuracy in positioning the target DNA. Single-molecule multiplex PCR was carried out to test the contamination. The results showed that the two overlapping DNA fragments isolated by AFM could be successfully sequenced with high quality and perfect contiguity, indicating that single-base resolution and long-coverage sequencing have been achieved simultaneously.
Subject Area	Instruments & Instrumentation ; Nuclear Science & Technology ; Physics
Indexed By	SCI
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000246590400001
Citation statistics	Cited Times:22[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/8190
Collection	中科院上海应用物理研究所2004-2010年
Corresponding Author	Lu, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation GB/T 7714	An, HJ,Huang, JH,Lu, M,et al. Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation[J]. NANOTECHNOLOGY,2007,18(22).
APA	An, HJ.,Huang, JH.,Lu, M.,Li, XL.,Lu, JH.,...&Lu, JH .(2007).Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation.NANOTECHNOLOGY,18(22).
MLA	An, HJ,et al."Single-base resolution and long-coverage sequencing based on single-molecule nanomanipulation".NANOTECHNOLOGY 18.22(2007).

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