CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells
Xu, DM(徐冬梅); Yao, SD(姚思德); Liu, YB; Sheng, KL(盛康龙); Hong, J; Gong, PJ(龚培军); Dong, L; Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationINTERNATIONAL JOURNAL OF PHARMACEUTICS
ISSN0378-5173
Volume338Issue:40545Pages:291
AbstractPolyethyleneimine nanogels (named as M-PEIs) with different sizes were prepared by photo-Fenton reaction in aqueous solution from which samples of 38, 75, 87, 121, 132 and 167 nm were selected for in vitro transfection. The homogeneous structure and the same component made it possible to study the size effect of M-PEIs nanogels on gene transfection efficiency when loading the same quantity of plasmid DNA (pLEGFP-C1) into A549, Be17402, BGC-823 and Hela cells. M-PEIs and its DNA complexes were characterized by photo correlation spectroscopy and atomic force microscopy. The protein expression was observed by flow cytometry and fluorescence microscopy. All of the DNA complexes had no obvious cytotoxicity and the surface charges were positive charged at the optimum weight ratio. Therefore, the expressed protein was affected by the size of M-PEIs when the same quantity of DNA was used to transfect cells. In addition, the samples of 75 and 87 nm yielded the highest transfection efficiency about 30% in all of the four cell lines which were also cell line independent. (c) 2007 Elsevier B.V. All rights reserved.
KeywordPolyethyleneimine (Pei) Gene Delivery Size Effect Nanogels Photochemistry
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000247494600039
Citation statistics
Cited Times:59[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8191
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorYao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Xu, DM,Yao, SD,Liu, YB,et al. Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2007,338(40545):291.
APA Xu, DM.,Yao, SD.,Liu, YB.,Sheng, KL.,Hong, J.,...&Yao, SD .(2007).Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells.INTERNATIONAL JOURNAL OF PHARMACEUTICS,338(40545),291.
MLA Xu, DM,et al."Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells".INTERNATIONAL JOURNAL OF PHARMACEUTICS 338.40545(2007):291.
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