CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Spectroscopic studies on the interaction between riboflavin and albumins
Zhao, HW(赵红卫); Ge, M(葛敏); Zhang, ZX(张朝霞); Wang, WF(王文锋); Wu, GZ(吴国忠); Zhao, HW (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2006
Source PublicationSPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
ISSN1386-1425
Volume65Issue:40606Pages:811
AbstractThe interactions between riboflavin (RF)and human and bovine serum albumin (HSA and BSA) were studied by using absorption and fluorescence spectroscopic methods. Intrinsic fluorescence emission spectra of serum albumin in the presence of RF show that the endogenous photosensitizer acts as a quencher. The decrease of fluorescence intensity at about 350 urn is attributed to changes in the environment of the protein fluorophores caused by the ligand. The quenching mechanisms of albumins by RF were discussed. The binding constants and binding site number were obtained at various temperatures. The distance between albumins and RF in the complexes suggests that the primary binding site for RF is close to tryptophan residue (Trp214) of HSA and Trp212 of BSA. The hydration process of albumins has also been discussed. (c) 2006 Elsevier B.V. All rights reserved.
KeywordRiboflavin Albumin Intrinsic Fluorescence Fluorescence Quenching
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000241785300048
Citation statistics
Cited Times:100[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8193
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorZhao, HW (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhao, HW,Ge, M,Zhang, ZX,et al. Spectroscopic studies on the interaction between riboflavin and albumins[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2006,65(40606):811.
APA Zhao, HW,Ge, M,Zhang, ZX,Wang, WF,Wu, GZ,&Zhao, HW .(2006).Spectroscopic studies on the interaction between riboflavin and albumins.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,65(40606),811.
MLA Zhao, HW,et al."Spectroscopic studies on the interaction between riboflavin and albumins".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 65.40606(2006):811.
Files in This Item:
File Name/Size DocType Version Access License
Spectroscopic studie(324KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, HW(赵红卫)]'s Articles
[Ge, M(葛敏)]'s Articles
[Zhang, ZX(张朝霞)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, HW(赵红卫)]'s Articles
[Ge, M(葛敏)]'s Articles
[Zhang, ZX(张朝霞)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, HW(赵红卫)]'s Articles
[Ge, M(葛敏)]'s Articles
[Zhang, ZX(张朝霞)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Spectroscopic studies on the interaction between riboflavin and albumins.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.