Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Stabilization of alpha-chymotrypsin by covalent immobilization on amine-functionalized superparamagnetic nanogel | |
| Hong, J（洪军）; Gong, PJ（龚培军）; Xu, DM（徐冬梅）; Dong, L; Yao, SD（姚思德）; Yao, S (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | JOURNAL OF BIOTECHNOLOGY
 |
| ISSN | 0168-1656 |
| Volume | 128Issue:3Pages:597 |
| Abstract | Stabilization of alpha-chymotrypsin (CT) by covalent immobilization on the amine-functionalized magnetic nanogel was studied. The amino groups containing superparamagnetic nanogel was obtained by Hoffman degradation of the polyacrylamide (PAM)-coated Fe3O4 nanoparticles prepared by facile photochemical in situ polymerization. CT was then covalently bound to the magnetic nanogel with reactive amino groups by using 1-ethyl-3-(3-dimethylaminepropyl) carbodiimide as coupling reagent. The binding capacity was determined to be 61 mg enzyme/g nanogel by BCA protein assay. Specific activity of the immobilized CT was measured to be 0.93 U/(mg min), 59.3% as that of free CT. The obtained immobilized enzyme had better resistance to temperature and pH inactivation in comparison to free enzyme and thus widened the ranges of reaction pH and temperature. The immobilized enzyme exhibited good thermostability, storage stability and reusability. Kinetic parameters were determined for both the immobilized and free enzyme. The value of K,, of the immobilized enzyme was larger than did the free form, whereas the V-max was smaller for the immobilized enzyme. (c) 2006 Elsevier B.V. All rights reserved. |
| Keyword | Amine-functionalized Superparamagnetic Nanogel Photochemical In Situ Polymerization Covalent Immobilization Alpha-chymotrypsin |
| Subject Area | Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000244404400016 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8194 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Yao, S (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Hong, J,Gong, PJ,Xu, DM,et al. Stabilization of alpha-chymotrypsin by covalent immobilization on amine-functionalized superparamagnetic nanogel[J]. JOURNAL OF BIOTECHNOLOGY,2007,128(3):597. |
| APA | Hong, J,Gong, PJ,Xu, DM,Dong, L,Yao, SD,&Yao, S .(2007).Stabilization of alpha-chymotrypsin by covalent immobilization on amine-functionalized superparamagnetic nanogel.JOURNAL OF BIOTECHNOLOGY,128(3),597. |
| MLA | Hong, J,et al."Stabilization of alpha-chymotrypsin by covalent immobilization on amine-functionalized superparamagnetic nanogel".JOURNAL OF BIOTECHNOLOGY 128.3(2007):597. |
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