CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA
Zhang, J; Song, SP(宋世平); Wang, LH(王丽华); Pan, D; Fan, CH(樊春海); Fan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationNATURE PROTOCOLS
ISSN1754-2189
Volume2Issue:11Pages:2888
AbstractWe report a protocol for the amplified detection of target DNA by using a chronocoulometric DNA sensor (CDS). Electrochemistry is known to be rapid, sensitive and cost-effective; it thus offers a promising approach for DNA detection. Our CDS protocol is based on a 'sandwich' detection strategy, involving a capture probe DNA immobilized on a gold electrode and a reporter probe DNA loaded on gold nanoparticles (AuNPs). Each probe flanks one of two fragments of the target sequence. A single DNA hybridization event brings AuNPs, along with hundreds of reporter probes, in the proximity of the electrode. We then employ chronocoulometry to interrogate [Ru(NH(3))(6)](3+) electrostatically bound to the captured DNA strands. This AuNP-amplified DNA sensor can selectively detect as low as femtomolar (zeptomoles) concentrations of DNA targets and conveniently analyze a breast cancer-associated BRCA-1 mutant DNA. The time range for the entire protocol is similar to 3 d, whereas the DNA sensing takes less than 2 h to complete.
Subject AreaMaterials Science ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000253140000026
Citation statistics
Cited Times:314[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8242
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorFan, CH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, J,Song, SP,Wang, LH,et al. A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA[J]. NATURE PROTOCOLS,2007,2(11):2888.
APA Zhang, J,Song, SP,Wang, LH,Pan, D,Fan, CH,&Fan, CH .(2007).A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA.NATURE PROTOCOLS,2(11),2888.
MLA Zhang, J,et al."A gold nanoparticle-based chronocoulometric DNA sensor for amplified detection of DNA".NATURE PROTOCOLS 2.11(2007):2888.
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