A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)

CAS OpenIR > 中科院上海应用物理研究所2004-2010年

	A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)
	Zhang, Y（张益）; Lu, JH; Li, MQ（李民乾）; Hu, J（胡钧）; Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
	2007
Source Publication	INTERNATIONAL JOURNAL OF NANOTECHNOLOGY
ISSN	1475-7435
Volume	4 Issue:40545 Pages:163
Abstract	In this mini-review we present the concept and the advancement of a novel strategy termed "ordered single molecule sequencing based on nanomanipulation (OsmSN)" for whole-genome sequencing. Techniques have been developed to integrate the processes including dissection and isolation of DNA molecules with sufficient spatial resolution together with the subsequent single molecule PCR to achieve the analysis of a particular DNA fragment from a genome DNA molecule. A nanoparticle-assisted PCR protocol was also developed to enhance the specificity and yield of PCR to meet the special requirement of the OsmSN.
Keyword	Dna Sequencing Ordered Single Molecule Sequencing Based On nanomanipulatiOn Osmsn Atomic Force Microscopy Afm Nano-dissection Isolation Single-molecule Pcr Nanoparticle Pcr
Subject Area	Environmental Sciences & Ecology ; Agriculture ; Water Resources
Indexed By	SCI
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000245674700011
Citation statistics	Cited Times:4[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/8246
Collection	中科院上海应用物理研究所2004-2010年
Corresponding Author	Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation GB/T 7714	Zhang, Y,Lu, JH,Li, MQ,et al. A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)[J]. INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,2007,4(40545):163.
APA	Zhang, Y,Lu, JH,Li, MQ,Hu, J,&Hu, J .(2007).A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN).INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,4(40545),163.
MLA	Zhang, Y,et al."A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)".INTERNATIONAL JOURNAL OF NANOTECHNOLOGY 4.40545(2007):163.

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