Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN) | |
| Zhang, Y（张益）; Lu, JH; Li, MQ（李民乾）; Hu, J（胡钧）; Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | INTERNATIONAL JOURNAL OF NANOTECHNOLOGY
 |
| ISSN | 1475-7435 |
| Volume | 4Issue:40545Pages:163 |
| Abstract | In this mini-review we present the concept and the advancement of a novel strategy termed "ordered single molecule sequencing based on nanomanipulation (OsmSN)" for whole-genome sequencing. Techniques have been developed to integrate the processes including dissection and isolation of DNA molecules with sufficient spatial resolution together with the subsequent single molecule PCR to achieve the analysis of a particular DNA fragment from a genome DNA molecule. A nanoparticle-assisted PCR protocol was also developed to enhance the specificity and yield of PCR to meet the special requirement of the OsmSN. |
| Keyword | Dna Sequencing Ordered Single Molecule Sequencing Based On nanomanipulatiOn Osmsn Atomic Force Microscopy Afm Nano-dissection Isolation Single-molecule Pcr Nanoparticle Pcr |
| Subject Area | Environmental Sciences & Ecology ; Agriculture ; Water Resources |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000245674700011 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8246 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, Y,Lu, JH,Li, MQ,et al. A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)[J]. INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,2007,4(40545):163. |
| APA | Zhang, Y,Lu, JH,Li, MQ,Hu, J,&Hu, J .(2007).A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN).INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,4(40545),163. |
| MLA | Zhang, Y,et al."A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)".INTERNATIONAL JOURNAL OF NANOTECHNOLOGY 4.40545(2007):163. |
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