CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)
Zhang, Y(张益); Lu, JH; Li, MQ(李民乾); Hu, J(胡钧); Hu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
2007
Source PublicationINTERNATIONAL JOURNAL OF NANOTECHNOLOGY
ISSN1475-7435
Volume4Issue:40545Pages:163
AbstractIn this mini-review we present the concept and the advancement of a novel strategy termed "ordered single molecule sequencing based on nanomanipulation (OsmSN)" for whole-genome sequencing. Techniques have been developed to integrate the processes including dissection and isolation of DNA molecules with sufficient spatial resolution together with the subsequent single molecule PCR to achieve the analysis of a particular DNA fragment from a genome DNA molecule. A nanoparticle-assisted PCR protocol was also developed to enhance the specificity and yield of PCR to meet the special requirement of the OsmSN.
KeywordDna Sequencing Ordered Single Molecule Sequencing Based On nanomanipulatiOn Osmsn Atomic Force Microscopy Afm Nano-dissection Isolation Single-molecule Pcr Nanoparticle Pcr
Subject AreaEnvironmental Sciences & Ecology ; Agriculture ; Water Resources
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000245674700011
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Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8246
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHu, J (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, POB 800-204, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Y,Lu, JH,Li, MQ,et al. A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)[J]. INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,2007,4(40545):163.
APA Zhang, Y,Lu, JH,Li, MQ,Hu, J,&Hu, J .(2007).A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN).INTERNATIONAL JOURNAL OF NANOTECHNOLOGY,4(40545),163.
MLA Zhang, Y,et al."A strategy for ordered single molecule sequencing based on nanomanipulation (OsmSN)".INTERNATIONAL JOURNAL OF NANOTECHNOLOGY 4.40545(2007):163.
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