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Crystal structure of human mu-crystallin complexed with NADPH
Cheng, ZJ; Sun, LH(孙丽华); He, JH(何建华); Gong, WM; Gong, WM (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
2007
Source PublicationPROTEIN SCIENCE
ISSN0961-8368
Volume16Issue:2Pages:329
AbstractHuman cytosolic 3,5,39-triiodo-L-thyronine-binding protein, also called mu-crystallin or CRYM, plays important physiological roles in transporting 3,5,39-triiodo-L-thyronine (T-3) into nuclei and regulating thyroid-hormone-related gene expression. The crystal structure of human CRYM's bacterial homolog Pseudomonas putida ornithine cyclodeaminase and Archaeoglobus fulgidus alanine dehydrogenase have been available, but no CRYM structure has been reported. Here, we report the crystal structure of human CRYM bound with NADPH refined to 2.6 angstrom, and there is one dimer in the asymmetric unit. The structure contains two domains: a Rossmann fold-like NADPH-binding domain and a dimerization domain. Different conformations of the loop Arg83-His92 have been observed in two monomers of human CRYM in the same asymmetric unit. The peptide bond of Val89-Pro90 is a trans-configuration in one monomer but a cis-configuration in the other. A detailed comparison of the human mu-crystallin structure with its structurally characterized homologs including the overall comparison and super-position of active sites was conducted. Finally, a putative T-3-binding site in human CRYM is proposed based on comparison with structural homologs.
KeywordMu-crystallin P38 Cytosolic 3 5 3 '-triiodo-l-thyronine-binding Protein Crystal Structure Cis/trans Isomerization Of Proline T-3-binding Site Nonsyndromic Deafness
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000243640600018
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8263
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorGong, WM (reprint author), Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Cheng, ZJ,Sun, LH,He, JH,et al. Crystal structure of human mu-crystallin complexed with NADPH[J]. PROTEIN SCIENCE,2007,16(2):329.
APA Cheng, ZJ,Sun, LH,He, JH,Gong, WM,&Gong, WM .(2007).Crystal structure of human mu-crystallin complexed with NADPH.PROTEIN SCIENCE,16(2),329.
MLA Cheng, ZJ,et al."Crystal structure of human mu-crystallin complexed with NADPH".PROTEIN SCIENCE 16.2(2007):329.
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