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| Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein | |
| Xu, C（徐春艳）; Song, J; Ding, Y; Yu, F; Sun, LH; Tang, L（唐琳）; Hu, XJ; Zhang, ZH; He, J（何建华）; Hu, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | PROTEIN AND PEPTIDE LETTERS
 |
| ISSN | 0929-8665 |
| Volume | 14Issue:5Pages:505 |
| Abstract | Sau3AI is a type II restriction endonuclease that recognizes the palindromic sequence 5'-GATC-3' and cleaves 5' to G residue on each strand. The E64A mutant full length protein was cloned and expressed in Escherichia coli. The purified (HiS)(6)-tagged protein has monomer and dimer fraction and was crystallized by the hanging-drop vapor-diffusion technique. The dimer protein crystals can diffract to 3.0 angstrom resolution and the monomer protein crystals can diffract to better than 2.8 angstrom resolution. One completed dataset has been collected and it shows that the monomer orthorhombic Sau3AIIE64A crystal is in space group C2221 with unit cell parameters (69.44, 197.60, 191.46, 90, 90, 90) and contains two molecules in one asymmetric unit. |
| Keyword | Sau3ai Endonuclease Crystallization Preliminary X-ray Analysis |
| Subject Area | Materials Science ; Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000247415500017 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8265 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Hu, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, C,Song, J,Ding, Y,et al. Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein[J]. PROTEIN AND PEPTIDE LETTERS,2007,14(5):505. |
| APA | Xu, C.,Song, J.,Ding, Y.,Yu, F.,Sun, LH.,...&Hu, XJ .(2007).Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein.PROTEIN AND PEPTIDE LETTERS,14(5),505. |
| MLA | Xu, C,et al."Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein".PROTEIN AND PEPTIDE LETTERS 14.5(2007):505. |
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