CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein
Xu, C(徐春艳); Song, J; Ding, Y; Yu, F; Sun, LH; Tang, L(唐琳); Hu, XJ; Zhang, ZH; He, J(何建华); Hu, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2007
Source PublicationPROTEIN AND PEPTIDE LETTERS
ISSN0929-8665
Volume14Issue:5Pages:505
AbstractSau3AI is a type II restriction endonuclease that recognizes the palindromic sequence 5'-GATC-3' and cleaves 5' to G residue on each strand. The E64A mutant full length protein was cloned and expressed in Escherichia coli. The purified (HiS)(6)-tagged protein has monomer and dimer fraction and was crystallized by the hanging-drop vapor-diffusion technique. The dimer protein crystals can diffract to 3.0 angstrom resolution and the monomer protein crystals can diffract to better than 2.8 angstrom resolution. One completed dataset has been collected and it shows that the monomer orthorhombic Sau3AIIE64A crystal is in space group C2221 with unit cell parameters (69.44, 197.60, 191.46, 90, 90, 90) and contains two molecules in one asymmetric unit.
KeywordSau3ai Endonuclease Crystallization Preliminary X-ray Analysis
Subject AreaMaterials Science ; Physics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000247415500017
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8265
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHu, XJ (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Xu, C,Song, J,Ding, Y,et al. Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein[J]. PROTEIN AND PEPTIDE LETTERS,2007,14(5):505.
APA Xu, C.,Song, J.,Ding, Y.,Yu, F.,Sun, LH.,...&Hu, XJ .(2007).Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein.PROTEIN AND PEPTIDE LETTERS,14(5),505.
MLA Xu, C,et al."Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein".PROTEIN AND PEPTIDE LETTERS 14.5(2007):505.
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