CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Effect of the thickness of cell ultrathin sections on the topographical contrast in atomic force Microscopy
Li, XH; Ji, T; Sun, JL; Zhang, CP; Hu, J(胡钧); Hu, J (reprint author), Shanghai Jiao Tong Univ, Nanobiol Lab, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China
2005
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume32Issue:6Pages:562
AbstractThe effect of the thickness of ultrathin sections on the topographical contrast in the images of atomic force microscopy (AFM) had been studied. Three different cell lines, Tca8113, C6 and ECV-304 were treated with conventional TEM fixation and embedment techniques. Cut by ultramicrotomy and collected with mica pieces, thin sections with different thickness were imaged by AFM on both sides. The images observed from the lower surfaces showed that the cell regions were always concave compared to the epoxy resin regions, and the concavity was increased with the increase of the ultrathin section thickness. Interestingly, the images from upper surfaces showed a peculiar convexness in the cell regions when the thickness is small, and achieved a maximum when the thickness was about 80 mu, and finally became concave when the thickness was larger than 120 nm. Statistic analysis showed that this trend was a general phenomenon. The relevant mechanism has been discussed.
KeywordSurface Morphology Topographical Contrast Ultrathin Section Atomic Force Microscopy
Subject AreaInstruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000230057000013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8275
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHu, J (reprint author), Shanghai Jiao Tong Univ, Nanobiol Lab, BioX Life Sci Res Ctr, Shanghai 200030, Peoples R China
Recommended Citation
GB/T 7714
Li, XH,Ji, T,Sun, JL,et al. Effect of the thickness of cell ultrathin sections on the topographical contrast in atomic force Microscopy[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2005,32(6):562.
APA Li, XH,Ji, T,Sun, JL,Zhang, CP,Hu, J,&Hu, J .(2005).Effect of the thickness of cell ultrathin sections on the topographical contrast in atomic force Microscopy.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,32(6),562.
MLA Li, XH,et al."Effect of the thickness of cell ultrathin sections on the topographical contrast in atomic force Microscopy".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 32.6(2005):562.
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