CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Effects of the silanized mica surface on protein crystallization
Tang, L(唐琳); Huang, YB; Liu, DQ; Li, JL; Mao, K; Liu, L; Cheng, ZJ; Gong, WM; Hu, J(胡钧); He, JH(何建华); He, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, 2019 Baojia Rd, Shanghai 201800, Peoples R China
2005
Source PublicationACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN0907-4449
Volume61Pages:53
AbstractA freshly cleaved mica surface silanized by 3-aminopropyl triethoxysilane is flat over a large area, displays a controlled degree of hydrophobicity and contains positive charges. In this paper, mica sheets silanized by this method have been used as crystallization surfaces for lysozyme, trichosanthin and three other proteins of unknown structure. Crystallization experiments have been carried out by the hanging-drop vapour-diffusion technique and the results indicate that the silanized mica surface can ameliorate the protein crystallization process considerably compared with a silanized glass cover slip control. For lysozyme on the silanized mica surface, the induction time required for crystal growth decreases markedly. For trichosanthin, the crystal size is obviously larger and the number of crystals grown is much lower. For the three proteins of unknown structure, the diffraction ability of the crystals is improved considerably.
Subject AreaChemistry
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000225795100007
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8276
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHe, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, 2019 Baojia Rd, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Tang, L,Huang, YB,Liu, DQ,et al. Effects of the silanized mica surface on protein crystallization[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2005,61:53.
APA Tang, L.,Huang, YB.,Liu, DQ.,Li, JL.,Mao, K.,...&He, JH .(2005).Effects of the silanized mica surface on protein crystallization.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,61,53.
MLA Tang, L,et al."Effects of the silanized mica surface on protein crystallization".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 61(2005):53.
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