CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Microarrays of DNA and protein integrated on microfluidic chip
Zhang, ZX(张志祥); Shen, Z; Zhao, H; Li, B(李宾); Song, SP(宋世平); Hu, J(胡钧); Lin, BC; Li, MQ(李民乾); Zhang, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2005
Source PublicationACTA CHIMICA SINICA
ISSN0567-7351
Volume63Issue:18Pages:1743
AbstractTo combine the advantages of high-throughput microarray and microfluidic chip technology, microarray was fabricated in channels. DNA and protein were arrayed on quartz slide, which was reversibly sealed by poly(dimethylsiloxane) (PDMS) slab containing pattern of channels. The hybridization carried out in channels indicated that target DNA and proteins were bound to their complementary DNA and protein probes specifically. In this method, PDMS conjugated with polylysine was contributed to imparting the wettability and decreasing unspecific adsorption of biomolecules. Our data demonstrated that the integrated chip speeded up the detection compared to microarrays, extended the parallel channels with serial properties and enhanced the throughput of probes and targets.
KeywordMicroarray Microfluidic Chip Modification Polylysine
Subject AreaPhysics
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000232089800020
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8316
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorZhang, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, ZX,Shen, Z,Zhao, H,et al. Microarrays of DNA and protein integrated on microfluidic chip[J]. ACTA CHIMICA SINICA,2005,63(18):1743.
APA Zhang, ZX.,Shen, Z.,Zhao, H.,Li, B.,Song, SP.,...&Zhang, ZX .(2005).Microarrays of DNA and protein integrated on microfluidic chip.ACTA CHIMICA SINICA,63(18),1743.
MLA Zhang, ZX,et al."Microarrays of DNA and protein integrated on microfluidic chip".ACTA CHIMICA SINICA 63.18(2005):1743.
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