CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
许凤华; 周伟; 胡伟青; 沈华; 于俊峰; 尹端沚
Source Publication同位素
Abstract以2-巯基乙醇作为抗体的还原剂,葡萄糖酸钠作为中间弱配体,在pH5.0的条件下,以SnCl2快速还原188ReO4-,进行了单克隆抗体IgG的直接标记。探讨了影响标记率的因素。结果显示,优化后的标记条件为pH5.0,室温下标记3 h,SnCl2用量2.66μmol,葡萄糖酸钠用量80μmol。该方法操作步骤简单方便,标记率较高,为94.4%±0.7%,标记物不需分离纯化,且有良好的体外稳定性,室温下可稳定保存8 h。
Keyword~(188)Re 直接标记 Igg
Indexed ByCNKI
Funding Project应物所项目组
Document Type期刊论文
Corresponding Author许凤华
Recommended Citation
GB/T 7714
许凤华,周伟,胡伟青,等. ~(188)Re直接标记抗体方法研究[J]. 同位素,2009(1).
APA 许凤华,周伟,胡伟青,沈华,于俊峰,&尹端沚.(2009).~(188)Re直接标记抗体方法研究.同位素(1).
MLA 许凤华,et al."~(188)Re直接标记抗体方法研究".同位素 .1(2009).
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