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Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization | |
Hong, J(洪军); Xu, DM(徐冬梅); Sun, HW(孙汉文); Gong, PJ(宫培军); Dong, L(董黎); Yao, SD(姚思德); Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
2007 | |
Source Publication | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
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ISSN | 0251-0790 |
Volume | 28Issue:1Pages:177 |
Abstract | In this paper, amine-functionalized superparamagnetic nanogel was obtained by Hoffman degradation of the PAM-coated Fe3O4 nanoparticles prepared by photochemical in situ polymerization. alpha-Chymotrypsin was successfully bound to the magnetic nanogel with amino groups in the presence of 1-ethyl-3 -(3-dimethylaminepropyl) carbodiimide (EDC). The immobilized enzyme was characterized by using photo correlation spectroscopy(PCS), transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, thermogravimetric-differential scanning calorimetry(TG-DSC) and Fourier transform infrared spectroscopy. Mean particle, size of the immobilized enzyme was measured to be 31 nm by PCS; the binding capacity was 69 mg enzyme/g nanogel determined by TG analysis, 61 mg enzyme/g nanogel determined by standard BCA. The crystalline structure of Fe3O4 was affected by xenon lamp irradiation and enzyme immobilization. Magnetic content of the immobilized enzyme was as high as 88%. Specific activity of the immobilized alpha-chymotrypsin was 0.93 U/min center dot mg, 59.3% of free form. It can be expected that the immobilized alpha-chymotrypsin has a potential in the fast detection and separation, as well as the reuse of immobilized enzymes. |
Keyword | Photochemical In Situ Polymerization Immobilized Enzyme Superparamagnetic Fe3o4 Alpha-chymotrypsin |
Subject Area | Physics |
Indexed By | SCI |
Language | 英语 |
Funding Project | 应物所项目组 |
WOS ID | WOS:000243581800044 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.sinap.ac.cn/handle/331007/8645 |
Collection | 中科院上海应用物理研究所2004-2010年 |
Corresponding Author | Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
Recommended Citation GB/T 7714 | Hong, J,Xu, DM,Sun, HW,et al. Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(1):177. |
APA | Hong, J.,Xu, DM.,Sun, HW.,Gong, PJ.,Dong, L.,...&Yao, SD .(2007).Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(1),177. |
MLA | Hong, J,et al."Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.1(2007):177. |
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