Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization | |
| Hong, J（洪军）; Xu, DM（徐冬梅）; Sun, HW（孙汉文）; Gong, PJ（宫培军）; Dong, L（董黎）; Yao, SD（姚思德）; Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China | |
| 2007 | |
| Source Publication | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| ISSN | 0251-0790 |
| Volume | 28Issue:1Pages:177 |
| Abstract | In this paper, amine-functionalized superparamagnetic nanogel was obtained by Hoffman degradation of the PAM-coated Fe3O4 nanoparticles prepared by photochemical in situ polymerization. alpha-Chymotrypsin was successfully bound to the magnetic nanogel with amino groups in the presence of 1-ethyl-3 -(3-dimethylaminepropyl) carbodiimide (EDC). The immobilized enzyme was characterized by using photo correlation spectroscopy(PCS), transmission electron microscopy (TEM), X-ray diffraction (XRD) analysis, thermogravimetric-differential scanning calorimetry(TG-DSC) and Fourier transform infrared spectroscopy. Mean particle, size of the immobilized enzyme was measured to be 31 nm by PCS; the binding capacity was 69 mg enzyme/g nanogel determined by TG analysis, 61 mg enzyme/g nanogel determined by standard BCA. The crystalline structure of Fe3O4 was affected by xenon lamp irradiation and enzyme immobilization. Magnetic content of the immobilized enzyme was as high as 88%. Specific activity of the immobilized alpha-chymotrypsin was 0.93 U/min center dot mg, 59.3% of free form. It can be expected that the immobilized alpha-chymotrypsin has a potential in the fast detection and separation, as well as the reuse of immobilized enzymes. |
| Keyword | Photochemical In Situ Polymerization Immobilized Enzyme Superparamagnetic Fe3o4 Alpha-chymotrypsin |
| Subject Area | Physics |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000243581800044 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8645 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Corresponding Author | Yao, SD (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China |
| Recommended Citation GB/T 7714 | Hong, J,Xu, DM,Sun, HW,et al. Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2007,28(1):177. |
| APA | Hong, J.,Xu, DM.,Sun, HW.,Gong, PJ.,Dong, L.,...&Yao, SD .(2007).Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,28(1),177. |
| MLA | Hong, J,et al."Photochemical synthesis and characterization of amine-functionalized superparamagnetic nanogel for alpha-chymotrypsin immobilization".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 28.1(2007):177. |
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