CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy
Zhang, F; Ji, LN; Tang, L(唐琳); Hu, J(胡钧); Hui, HY; Xu, HJ(徐洪杰); He, JH(何建华); He, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
2005
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN1672-9145
Volume37Issue:2Pages:113
AbstractHuman alpha-synuclein is a presynaptic terminal protein and can form insoluble fibrils that are believed to play an important role in the pathogenesis of several neurodegenerative diseases such as Parkinson's disease, dementia with Lewy bodies and Lewy body variant of Alzheimer's disease. In this paper, in situ atomic force microscopy has been used to study the structural properties of alpha-synuclein fibrils in solution using two different atomic force microscopy imaging modes: tapping mode and contact mode. In the in situ contact mode atomic force microscopy experiments alpha-synuclein fibrils quickly broke into fragments, and a similar phenomenon was found using tapping mode atomic force microscopy in which alpha-synuclein fibrils were incubated with guanidine hydrochloride (0.6 M). The alpha-synuclein fibrils kept their original filamentous topography for over I h in the in situ tapping mode atomic force microscopy experiments. The present results provide indirect evidence on how beta-sheets assemble into alpha-synuclein fibrils on a nanometer scale.
KeywordAlpha-synuclein Atomic Force Microscopy Tapping Mode Contact Mode Guanidine Hydrochloride
Subject AreaBiochemistry & Molecular Biology
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000226917100005
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/8673
Collection中科院上海应用物理研究所2004-2010年
Corresponding AuthorHe, JH (reprint author), Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai 201800, Peoples R China
Recommended Citation
GB/T 7714
Zhang, F,Ji, LN,Tang, L,et al. Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2005,37(2):113.
APA Zhang, F.,Ji, LN.,Tang, L.,Hu, J.,Hui, HY.,...&He, JH .(2005).Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,37(2),113.
MLA Zhang, F,et al."Structural evidence for alpha-synuclein fibrils using in situ atomic force microscopy".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 37.2(2005):113.
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