Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A Gold Nanoparticle-Based Microfluidic Protein Chip for Tumor Markers | |
| Yan, J; Pan, D; Zhu, CF; Wang, LH（王丽华）; Song, SP（宋世平）; Fan, CH（樊春海） | |
| 2009 | |
| Conference Name | 6th International Conference on Nanoscience and Technology |
| Source Publication | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY |
| Pages | 1194 |
| Conference Date | JUN 04-06, 2007 |
| Conference Place | Beijing, PEOPLES R CHINA |
| Abstract | A cancer protein chip has been developed incorporating with signal amplification technology based on gold nanoparticles (AuNPs) and microfluidic technology. The chip was a reversed phase one that deposited the target proteins directly onto chip surface. At first, a tumor marker protein, carcinoma embryonic antigen (CEA), was captured on the chip with the help of first layer microfluidic channel. Then, monoclonal antibody was reacted with the CEA through the second layer microfluidic channel at upright direction against the first layer. In the end, AuNPs labeled secondary antibody and silver enhancement regent were added to creating positive or negative signals in crossed areas, depending on the amounts of tumor marker protein. Using the protein chip based on nanotechnology, we could detect CEA at a wide range of concentration (5-50000 ng/mL) and also had high sensitivity (LOD < 1 pM). Because of its quick detection procedure and no need for high-cost instruments, the protein chip showed potential use for point-of-care (POC) diagnostics. |
| ISSN | 1533-4880 |
| Indexed By | SCI |
| Funding Project | 应物所课题组 |
| Language | 英语 |
| Document Type | 会议论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/8811 |
| Collection | 中科院上海应用物理研究所2004-2010年 |
| Recommended Citation GB/T 7714 | Yan, J,Pan, D,Zhu, CF,et al. A Gold Nanoparticle-Based Microfluidic Protein Chip for Tumor Markers[C],2009:1194. |
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| A Gold Nanoparticle-（11860KB） | 开放获取 | -- | View Download | |||
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