CAS OpenIR  > 中科院上海应用物理研究所2004-2010年
Ligase-based multiple DNA analysis by using an electrochemical sensor array
Wan, Y; Zhang, J; Liu, G; Pan, D; Wang, LH(王丽华); Song, SP(宋世平); Fan, CH(樊春海)
Conference Name10th World Congress on Biosensors
Conference DateMAY 14-16, 2008
Conference PlaceShanghai, PEOPLES R CHINA
AbstractWe herein report an electrochemical biosensor for the sequence-specific detection of DNA with high discrimination ability for single-nucleotide polymorphisms (SNPs). This DNA sensor was constructed by a pair of flanking probes that "sandwiched" the target. A 16-electrode electrochemical sensor array was employed, each having one individual DNA capture probe immobilized at gold electrodes via gold-thiol chemistry. By coupling with a biotin-tagged detection probe, we were able to detect multiple DNA targets with a single array. In order to realize SNP detection, a ligase-based approach was employed. In this method, both the capture probe and the detection probe were in tandem upon being hybridized with the target. Importantly, we employed a ligase that specifically could ligate tandem sequences only in the absence of mismatches. As a result, when both probes were complementary to the target, they were ligated in the presence of the ligase, thus being retained at the surface during the subsequent stringent washing steps. In contrast, if there existed 1-base mismatch, which could be efficiently recognized by the ligase, the detection probe was not ligated and subsequently washed away. A conjugate of avidin-horseradish peroxidase was then attached to the biotin label at the end of the detection probe via the biotin-avidin bridge. We then electrochemically interrogated the electrical current for the peroxidase-catalyzed reduction of hydrogen peroxide. We demonstrated that the electrochemical signal for the wild-type DNA was significantly larger than that for the sequence harboring the SNP. (c) 2008 Elsevier B.V. All rights reserved.
Indexed BySCI
Funding Project应物所课题组
Document Type会议论文
Recommended Citation
GB/T 7714
Wan, Y,Zhang, J,Liu, G,et al. Ligase-based multiple DNA analysis by using an electrochemical sensor array[C],2009:1209.
Files in This Item: Download All
File Name/Size DocType Version Access License
Ligase-basedmultiple(417KB) 开放获取--View Download
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wan, Y]'s Articles
[Zhang, J]'s Articles
[Liu, G]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wan, Y]'s Articles
[Zhang, J]'s Articles
[Liu, G]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wan, Y]'s Articles
[Zhang, J]'s Articles
[Liu, G]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Ligase-basedmultipleDNAanalysis by using an electrochemicalsensorarray.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.