CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis
Zhu, Changfeng; Wen, Yanqin; Peng, Hongzhen(彭红珍); Long, Yitao; He, Yao; Huang, Qing(黄庆); Li, Di(李迪); Fan, Chunhai(樊春海)
2011
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume399Issue:10Pages:3459
AbstractThe operation of DNA nanomachines is generally triggered by either conformational changes of DNA nanostructure or external environmental stimuli. In the present study, we demonstrate an alternative driving force, DNA methylation, to stimulate DNA machine operation. DNA methylation changes neither DNA sequence and conformation nor external environment, however, blocks its cleavage by corresponding methylation-sensitive restriction endonuclease. We thus designed a strand displacement amplification DNA machine, which could be stimulated upon DNA methylation and then autonomously generates accumulated amounts of peroxidase-mimicking DNAzyme signaling machine products in an isothermal manner. The machine product DNAzyme could catalyze the H(2)O(2)-mediated oxidation of 2,2'-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS(2-)) to a colored product ABTS(center dot-). This methylation-stimulated DNA machine was further used as a colorimetric assay for analysis of methyltransferases activities and screening of methylation inhibitors. As compared with classical methylation assay, this facile isothermal DNA machine avoids the introduction of methylation-specific polymerase chain reaction and radioactive labels, which might be employed as an effective tool for DNA methylation analysis.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000288398700016
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9450
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhu, Changfeng,Wen, Yanqin,Peng, Hongzhen,et al. A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3459.
APA Zhu, Changfeng.,Wen, Yanqin.,Peng, Hongzhen.,Long, Yitao.,He, Yao.,...&Fan, Chunhai.(2011).A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(10),3459.
MLA Zhu, Changfeng,et al."A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.10(2011):3459.
Files in This Item:
File Name/Size DocType Version Access License
A methylation-stimul(327KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhu, Changfeng]'s Articles
[Wen, Yanqin]'s Articles
[Peng, Hongzhen(彭红珍)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhu, Changfeng]'s Articles
[Wen, Yanqin]'s Articles
[Peng, Hongzhen(彭红珍)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhu, Changfeng]'s Articles
[Wen, Yanqin]'s Articles
[Peng, Hongzhen(彭红珍)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: A methylation-stimulated DNA machine an autonomous isothermal route to methyltransferase activity and inhibition analysis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.