Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis | |
| Zhu, Changfeng; Wen, Yanqin; Peng, Hongzhen（彭红珍）; Long, Yitao; He, Yao; Huang, Qing（黄庆）; Li, Di（李迪）; Fan, Chunhai（樊春海） | |
| 2011 | |
| Source Publication | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| ISSN | 1618-2642 |
| Volume | 399Issue:10Pages:3459 |
| Abstract | The operation of DNA nanomachines is generally triggered by either conformational changes of DNA nanostructure or external environmental stimuli. In the present study, we demonstrate an alternative driving force, DNA methylation, to stimulate DNA machine operation. DNA methylation changes neither DNA sequence and conformation nor external environment, however, blocks its cleavage by corresponding methylation-sensitive restriction endonuclease. We thus designed a strand displacement amplification DNA machine, which could be stimulated upon DNA methylation and then autonomously generates accumulated amounts of peroxidase-mimicking DNAzyme signaling machine products in an isothermal manner. The machine product DNAzyme could catalyze the H(2)O(2)-mediated oxidation of 2,2'-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS(2-)) to a colored product ABTS(center dot-). This methylation-stimulated DNA machine was further used as a colorimetric assay for analysis of methyltransferases activities and screening of methylation inhibitors. As compared with classical methylation assay, this facile isothermal DNA machine avoids the introduction of methylation-specific polymerase chain reaction and radioactive labels, which might be employed as an effective tool for DNA methylation analysis. |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | 应物所项目组 |
| WOS ID | WOS:000288398700016 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/9450 |
| Collection | 中科院上海应用物理研究所2011-2020年 |
| Recommended Citation GB/T 7714 | Zhu, Changfeng,Wen, Yanqin,Peng, Hongzhen,et al. A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3459. |
| APA | Zhu, Changfeng.,Wen, Yanqin.,Peng, Hongzhen.,Long, Yitao.,He, Yao.,...&Fan, Chunhai.(2011).A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(10),3459. |
| MLA | Zhu, Changfeng,et al."A methylation-stimulated DNA machine: an autonomous isothermal route to methyltransferase activity and inhibition analysis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.10(2011):3459. |
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