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Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides
Tang, Zhuzhao; Chen, Haode; Song, Shiping(宋世平); Fan, Chunhai(樊春海); Zhang, Dabing; Wu, Aibo
2011
Source PublicationJOURNAL OF AOAC INTERNATIONAL
ISSN1060-3271
Volume94Issue:1Pages:307
AbstractRecombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 mu g MWCNT and 5 mu L enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion (R), 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 mu g/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5-5 mu g/L and 73.4 to 118.4% for carbofuran at 1-10 mu g/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000287222000035
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9492
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Tang, Zhuzhao,Chen, Haode,Song, Shiping,et al. Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides[J]. JOURNAL OF AOAC INTERNATIONAL,2011,94(1):307.
APA Tang, Zhuzhao,Chen, Haode,Song, Shiping,Fan, Chunhai,Zhang, Dabing,&Wu, Aibo.(2011).Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides.JOURNAL OF AOAC INTERNATIONAL,94(1),307.
MLA Tang, Zhuzhao,et al."Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides".JOURNAL OF AOAC INTERNATIONAL 94.1(2011):307.
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