CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure
Ge ZhiLei; Pei Hao; Wang LiHua(王丽华); Song ShiPing(宋世平); Fan ChunHai(樊春海)
2011
Source PublicationSCIENCE CHINA-CHEMISTRY
ISSN1674-7291
Volume54Issue:8Pages:1273
AbstractAn electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000294062100013
Citation statistics
Cited Times:70[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9503
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Ge ZhiLei,Pei Hao,Wang LiHua,et al. Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure[J]. SCIENCE CHINA-CHEMISTRY,2011,54(8):1273.
APA Ge ZhiLei,Pei Hao,Wang LiHua,Song ShiPing,&Fan ChunHai.(2011).Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure.SCIENCE CHINA-CHEMISTRY,54(8),1273.
MLA Ge ZhiLei,et al."Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure".SCIENCE CHINA-CHEMISTRY 54.8(2011):1273.
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