Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection

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	Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection
	Xu, Fenghua（许凤华）; Lei, Du; Du, Xiaoxia; Zhang, Chunfu（张春富）; Xie, Xuan; Yin, Duanzhi（尹端沚）
	2011
Source Publication	CONTRAST MEDIA & MOLECULAR IMAGING
ISSN	1555-4309
Volume	6 Issue:1 Pages:46
Abstract	One of the challenges facing superparamagnetic iron oxide (SPIO) nanoparticles is to improve their biological compatibility. While highly uniform SPIOs can be manufactured, the surfaces are hydrophobic as a result of the surfactants used in their fabrication. In this study, we developed a general strategy to fabricate an MR molecular imaging probe in one step by replacing hydrophobic surfactants with small peptides terminated with cysteine. The hydrophobic SPIO surface was transformed into a hydrophilic one by exchanging surface oleic acids with the peptides RGD-Cys or RGD-PEG-Cys. After the RGD-Cys and RGD-PEG-Cys peptide exchange, both RGD-Cys-SPIO and RGD-PEG-Cys-SPIO specifically targeted alpha(v)beta(3)-expressing cells (A549) in vitro, with RGD-Cys-SPIO achieving this more efficiently. Furthermore, MR imaging of A549 tumors receiving RGD-Cys-SPIO or RGD-PEG-Cys-SPIO demonstrated that both the targeted particles could reach and label the alpha(v)beta(3)-expressing tumor, much more efficiently than the non-targeted particles (Cys-SPIO). Histology showed that the probes not only target the tumor neovasculature but also extravasate from vessels and address the tumor cells. Our study shows that directly replacing oleic acid with cysteine or cysteine-terminated small peptides is a general strategy to transforming the hydrophobic surface of SPIO into a hydrophilic one, as well as providing targeting ligands. Such SPIOs are of interest as MR molecular imaging probes to detect for cancer in vivo. Copyright (C) 2010 John Wiley & Sons, Ltd.
Indexed By	SCI
Language	英语
Funding Project	应物所项目组
WOS ID	WOS:000287939100006
Citation statistics	Cited Times:9[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.sinap.ac.cn/handle/331007/9550
Collection	中科院上海应用物理研究所2011-2020年
Recommended Citation GB/T 7714	Xu, Fenghua,Lei, Du,Du, Xiaoxia,et al. Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection[J]. CONTRAST MEDIA & MOLECULAR IMAGING,2011,6(1):46.
APA	Xu, Fenghua,Lei, Du,Du, Xiaoxia,Zhang, Chunfu,Xie, Xuan,&Yin, Duanzhi.(2011).Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection.CONTRAST MEDIA & MOLECULAR IMAGING,6(1),46.
MLA	Xu, Fenghua,et al."Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection".CONTRAST MEDIA & MOLECULAR IMAGING 6.1(2011):46.

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