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Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection
Xu, Fenghua(许凤华); Lei, Du; Du, Xiaoxia; Zhang, Chunfu(张春富); Xie, Xuan; Yin, Duanzhi(尹端沚)
2011
Source PublicationCONTRAST MEDIA & MOLECULAR IMAGING
ISSN1555-4309
Volume6Issue:1Pages:46
AbstractOne of the challenges facing superparamagnetic iron oxide (SPIO) nanoparticles is to improve their biological compatibility. While highly uniform SPIOs can be manufactured, the surfaces are hydrophobic as a result of the surfactants used in their fabrication. In this study, we developed a general strategy to fabricate an MR molecular imaging probe in one step by replacing hydrophobic surfactants with small peptides terminated with cysteine. The hydrophobic SPIO surface was transformed into a hydrophilic one by exchanging surface oleic acids with the peptides RGD-Cys or RGD-PEG-Cys. After the RGD-Cys and RGD-PEG-Cys peptide exchange, both RGD-Cys-SPIO and RGD-PEG-Cys-SPIO specifically targeted alpha(v)beta(3)-expressing cells (A549) in vitro, with RGD-Cys-SPIO achieving this more efficiently. Furthermore, MR imaging of A549 tumors receiving RGD-Cys-SPIO or RGD-PEG-Cys-SPIO demonstrated that both the targeted particles could reach and label the alpha(v)beta(3)-expressing tumor, much more efficiently than the non-targeted particles (Cys-SPIO). Histology showed that the probes not only target the tumor neovasculature but also extravasate from vessels and address the tumor cells. Our study shows that directly replacing oleic acid with cysteine or cysteine-terminated small peptides is a general strategy to transforming the hydrophobic surface of SPIO into a hydrophilic one, as well as providing targeting ligands. Such SPIOs are of interest as MR molecular imaging probes to detect for cancer in vivo. Copyright (C) 2010 John Wiley & Sons, Ltd.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000287939100006
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9550
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Xu, Fenghua,Lei, Du,Du, Xiaoxia,et al. Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection[J]. CONTRAST MEDIA & MOLECULAR IMAGING,2011,6(1):46.
APA Xu, Fenghua,Lei, Du,Du, Xiaoxia,Zhang, Chunfu,Xie, Xuan,&Yin, Duanzhi.(2011).Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection.CONTRAST MEDIA & MOLECULAR IMAGING,6(1),46.
MLA Xu, Fenghua,et al."Modification of MR molecular imaging probes with cysteine-terminated peptides and their potential for in vivo tumour detection".CONTRAST MEDIA & MOLECULAR IMAGING 6.1(2011):46.
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