CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe
Zhang Xiao-Ping; Huang Yan-Qin; Fan Chun-Hai(樊春海); Huang Wei
2011
Source PublicationCHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN0251-0790
Volume32Issue:11Pages:2548
AbstractA novel method was developed for phosphatase detection based on cationic conjugated polymer (CCP) and enzymatic substrate. The CCP associated with the fluorescein-labeled anionic adenosine triphosphate( ATP) by electrostatic attractions and fluorescence resonance energy transfer( FRET) from the CCP to the fluorescein-labeled ATP occurred. When phosphatase was added, it catalyzed the hydrolysis of phosphate groups from its substrate, ATP, and the final product adenosine was neutral, which made the CCP detached from adenosine. Then the FRET efficiency decreased. The results show that the degree of FRET efficiency decrease is related to the concentration of phosphatase. This detection method has the advantages including easy operations, real-time response, and high sensitivity. It can also sensitively assay other enzymes that promote the changes of the charge density of substrates; moreover, it provides a promising application in drug screening based on the inhibition of enzymatic activity.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000297231500016
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9558
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Zhang Xiao-Ping,Huang Yan-Qin,Fan Chun-Hai,et al. Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2011,32(11):2548.
APA Zhang Xiao-Ping,Huang Yan-Qin,Fan Chun-Hai,&Huang Wei.(2011).Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,32(11),2548.
MLA Zhang Xiao-Ping,et al."Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 32.11(2011):2548.
Files in This Item: Download All
File Name/Size DocType Version Access License
Novel Method of Phos(696KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang Xiao-Ping]'s Articles
[Huang Yan-Qin]'s Articles
[Fan Chun-Hai(樊春海)]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang Xiao-Ping]'s Articles
[Huang Yan-Qin]'s Articles
[Fan Chun-Hai(樊春海)]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang Xiao-Ping]'s Articles
[Huang Yan-Qin]'s Articles
[Fan Chun-Hai(樊春海)]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Novel Method of Phosphatase Detection Based on Cationic Conjugated Polymer and Enzymatic Substrate Probe.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.