CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy
Chen, Guangcun; Chen, Xincai; Yang, Yuanqiang; Hay, Anthony G.; Yu, Xiaohan(余笑寒); Chen, Yingxu
2011
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume77Issue:14Pages:4719
AbstractThe spatial and temporal distribution of metals in unsaturated Pseudomonas putida CZ1 biofilms was determined using synchrotron-based X-ray fluorescence microscopy (XRF). It was found that Fe, Mn, and Ca were mainly distributed near the air-biofilm interface of a biofilm grown on 40 mM citrate, while there were two Fe-, Mn-, and Ca-rich layers within a biofilm grown on 10 mM citrate. The sorption of copper by biofilm grown in medium containing 10 mM citrate was rapid, with copper being found throughout the biofilm after only 1 h of exposure. Copper initially colocalized with Fe and Mn element layers in the biofilm and then precipitated in a 40-mu m-thick layer near the air-biofilm interface when exposed for 12 h. Cu K-edge X-ray absorption near edge structure (XANES) analysis revealed that Cu was primarily bound with citrate within the biofilm, and the precipitate formed in the biofilm exposed to copper for 12 h was most similar to copper phosphate. LIVE/DEAD staining revealed that cells at the biofilm-membrane interface were mostly alive even when the copper concentration reached 80.5 mg copper g(-1) biomass. This suggests that the biofilm matrix provided significant protection for cells in this area. These results significantly improve our understanding of metal acquisition, transportation, and immobilization in unsaturated biofilm systems.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000292510400003
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9591
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Chen, Guangcun,Chen, Xincai,Yang, Yuanqiang,et al. Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2011,77(14):4719.
APA Chen, Guangcun,Chen, Xincai,Yang, Yuanqiang,Hay, Anthony G.,Yu, Xiaohan,&Chen, Yingxu.(2011).Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,77(14),4719.
MLA Chen, Guangcun,et al."Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.14(2011):4719.
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