CAS OpenIR  > 中科院上海应用物理研究所2011-2019年
Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins
Li, Jiang; Wan, Ying; Wang, Lihua(王丽华); Zhu, Xinhua; Su, Yan; Li, Di(李迪); Zhao, Yun; Huang, Qing(黄庆); Song, Shiping(宋世平); Fan, Chunhai(樊春海)
2011
Source PublicationANALYTICA CHIMICA ACTA
ISSN0003-2670
Volume702Issue:1Pages:114
AbstractIn this report, we developed a universal assay method for both genomic DNA and proteins by using enzyme-based multi-component optical nanoprobes. The nanoprobes are gold nanoparticles assembled with bio-recognizing and signaling elements. We firstly demonstrated that the nanoprobes could detect unpurified asymmetric polymerase chain reaction (PCR) product from genomic DNA of Escherichia coil, with the sensitivity approximately 10 times higher than that of quantitative real-time PCR assay. The limit of detection CLOD) of our nanoprobe-based method is less than 10 pg template DNA (target DNA). Using DNA aptamers as recognition elements, we also showed that as few as 0.1 nM thrombin could be colorimetrically detected with high specificity. These results indicated that the enzyme-based multicomponent nanoprobes have the capability to work with real biological samples, and have the potential in various biological and clinical applications. (C) 2011 Elsevier B.V. All rights reserved.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000295245200014
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9612
Collection中科院上海应用物理研究所2011-2019年
Recommended Citation
GB/T 7714
Li, Jiang,Wan, Ying,Wang, Lihua,et al. Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins[J]. ANALYTICA CHIMICA ACTA,2011,702(1):114.
APA Li, Jiang.,Wan, Ying.,Wang, Lihua.,Zhu, Xinhua.,Su, Yan.,...&Fan, Chunhai.(2011).Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins.ANALYTICA CHIMICA ACTA,702(1),114.
MLA Li, Jiang,et al."Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins".ANALYTICA CHIMICA ACTA 702.1(2011):114.
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