CAS OpenIR  > 中科院上海原子核所2003年前
A peptide motif consisting of glycine, alanine, and valine is required for the fibrillization and cytotoxicity of human alpha-synuclein
Du, HN; Tang, L(唐琳); Luo, XY; Li, HT(李洪涛); Hu, J(胡钧); Zhou, JW; Hu, HY
2003
Source PublicationBIOCHEMISTRY
ISSN0006-2960
Volume42Issue:29Pages:8870
AbstractAmyloid-like aggregation or fibrillization of alpha-synuclein (alpha-Syn) and the filamentous deposits in Lewy bodies are believed to be closely associated with several fatal neurodegenerative disorders, including Parkinson's disease and Alzheimer's disease. Here, we report the importance of a nine-residue peptide Motif, (66)VGGAVVTGV(74), in the fibrillization and cytotoxicity of human alpha-Syn. Mutagenesis combined with thioflavin T fluorescence detection, atomic force microscopic imaging, and cytotoxicity assays reveal that deletion of this sequence completely eliminates alpha-Syn fibrillization and cell toxicity. However, deletion of the (71)VTGV(74) sequence decreases the fibrillization rate while the cytotoxicity remains unchanged. Incorporation of charged residues within this region slows aggregation and even impedes filament formation. In addition, substitution of Gly68 with Ala or C-terminal truncations of alpha-Syn accelerate the fibrillization processes. Circular dichroism studies suggest that beta-sheet formation is often concomitant with filament formation. Thus, this segment, namely, the GAV motif, is responsible for aggregation or fibrillization of alpha-Syn and perhaps other amyloidogenic proteins. The oligomers formed during fibrillogenesis might be associated with the cytotoxicities of various alpha-Syn species. This finding may provide further insight into the understanding of the molecular mechanism underlying the fibrillogenesis implicated in neurodegeneration as well as aid in drug design and development of transgenic models.
Indexed BySCI
Language英语
Funding Project应物所项目组
WOS IDWOS:000184390600025
Citation statistics
Cited Times:124[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9849
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
Du, HN,Tang, L,Luo, XY,et al. A peptide motif consisting of glycine, alanine, and valine is required for the fibrillization and cytotoxicity of human alpha-synuclein[J]. BIOCHEMISTRY,2003,42(29):8870.
APA Du, HN.,Tang, L.,Luo, XY.,Li, HT.,Hu, J.,...&Hu, HY.(2003).A peptide motif consisting of glycine, alanine, and valine is required for the fibrillization and cytotoxicity of human alpha-synuclein.BIOCHEMISTRY,42(29),8870.
MLA Du, HN,et al."A peptide motif consisting of glycine, alanine, and valine is required for the fibrillization and cytotoxicity of human alpha-synuclein".BIOCHEMISTRY 42.29(2003):8870.
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