CAS OpenIR  > 中科院上海原子核所2003年前
ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA
HANSMA, HG; SINSHEIMER, RL; LI, MQ(李民乾); HANSMA, PK
1992
Source PublicationNUCLEIC ACIDS RESEARCH
ISSN0305-1048
Volume20Issue:14Pages:3585
AbstractA method has been developed for imaging single-stranded DNA with the atomic force microscope (AFM). PHI-X174 single-stranded DNA in formaldehyde on mica can be imaged in the AFM under propanol or butanol or in air. Measured lengths of most molecules are on the order of 1-mu, although occasionally more extended molecules with lengths of 1.7 to 1.9-mu are seen. Single-stranded DNA in the AFM generally appears lumpier than double-stranded DNA, even when extended. Images of double-stranded lambda DNA in the AFM show more sharp kinks and bends than are typically observed in the electron microscope. Dense, aggregated fields of double-stranded plasmids can be converted by gentle rinsing with hot water to well spread fields.
Indexed BySCI
Language英语
Funding Project应物所项目组
Document Type期刊论文
Identifierhttp://ir.sinap.ac.cn/handle/331007/9874
Collection中科院上海原子核所2003年前
Recommended Citation
GB/T 7714
HANSMA, HG,SINSHEIMER, RL,LI, MQ,et al. ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA[J]. NUCLEIC ACIDS RESEARCH,1992,20(14):3585.
APA HANSMA, HG,SINSHEIMER, RL,LI, MQ,&HANSMA, PK.(1992).ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA.NUCLEIC ACIDS RESEARCH,20(14),3585.
MLA HANSMA, HG,et al."ATOMIC FORCE MICROSCOPY OF SINGLE-STRANDED AND DOUBLE-STRANDED DNA".NUCLEIC ACIDS RESEARCH 20.14(1992):3585.
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