Knowledge Management System Of Shanghai Institute of Applied Physics, CAS
| ATOMIC FORCE MICROSCOPY OF UNCOATED PLASMID DNA - NANOMETER RESOLUTION WITH ONLY NANOGRAM AMOUNTS OF SAMPLE | |
| LI, MQ（李民乾）; HANSMA, HG; VESENKA, J; KELDERMAN, G; HANSMA, PK | |
| 1992 | |
| Source Publication | JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
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| ISSN | 0739-1102 |
| Volume | 10Issue:3Pages:607 |
| Abstract | Reproducible, high-contrast, nanometer-resolution AFM images of uncoated plasmid DNA can be obtained with nanogram quantities of DNA with the help of two advances in sample preparation: (1) Heating a DNA solution at 35-degrees-C for 10 to 20 minutes before deposition on mica helps separate and spread the DNA, and (2) Using 5 microliter drops of die heated DNA solution in the concentration range of 2 to 10 nanogram/microliter in contact with a specially prepared mica surface for 5 to 10 minutes gives optimal coverage with only nanograms of DNA. |
| Indexed By | SCI |
| Funding Project | 应物所项目组 |
| Document Type | 期刊论文 |
| Identifier | http://ir.sinap.ac.cn/handle/331007/9875 |
| Collection | 中科院上海原子核所2003年前 |
| Recommended Citation GB/T 7714 | LI, MQ,HANSMA, HG,VESENKA, J,et al. ATOMIC FORCE MICROSCOPY OF UNCOATED PLASMID DNA - NANOMETER RESOLUTION WITH ONLY NANOGRAM AMOUNTS OF SAMPLE[J]. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,1992,10(3):607. |
| APA | LI, MQ,HANSMA, HG,VESENKA, J,KELDERMAN, G,&HANSMA, PK.(1992).ATOMIC FORCE MICROSCOPY OF UNCOATED PLASMID DNA - NANOMETER RESOLUTION WITH ONLY NANOGRAM AMOUNTS OF SAMPLE.JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS,10(3),607. |
| MLA | LI, MQ,et al."ATOMIC FORCE MICROSCOPY OF UNCOATED PLASMID DNA - NANOMETER RESOLUTION WITH ONLY NANOGRAM AMOUNTS OF SAMPLE".JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 10.3(1992):607. |
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